Effects of Nerve Growth Factor on\= Excitatory Amino Acid Content in\= Spinal Cord Neuron after
Spinal Cord Injury |
| |
Authors: | Cao Xiaojian\ Luo Yongxiang |
| |
Institution: | Cao Xiaojian\ Luo Yongxiang Department of Orthopedics,the First
Affiliated Hospital,Nanjing Medical University,\= Nanjing 210029,P.R.China\= \ 1Department of
Orthopedics,the Tongji Hospital,Tongji Medical University,\= Wuhan 430000,P.R.Chi |
| |
Abstract: | To investigate the protective mechanism of nerve growth factor (NGF) on spinal cord
injury (SCI), surgical spinal cord injury in Wistar rats was performed by a 10 g2.5 cm impact on
the posterior spinal cord at T8 level, and a thin plastic tube was placed in subarachoid space
below the injury level for perfusion of solution. To the experimental animals were given 60 g (20
l liquid) NGF, purified from bovine seminal plasma, at the moment of injury and 1,2,3,4,8,12,24
h after injury. An equal volume of normal saline was given to rats of the control group at the
same time. In the expermental group, the injured spinal cord tissue was taken following
treatment. The contents of the excitatory amino acids (Glu, Asp) were determined by high
performance liquid chromatography (HPLC). Excitatory amino acid contents in the injured spinal
cord were significantly increased at 10 min and 8 h after the injury as compared with those in
the control group. However, The peak values of the excitatory amino acid contents in NGF group
were obviously lowered. NGF might protect spinal cord against injury in vivo. One of the
possible mechanisms is that NGF prohibits neurotoxicity of the exitatory amino acids. |
| |
Keywords: | spinal cord injury nerve growth factor excitatory amino acid |
本文献已被 CNKI 等数据库收录! |
|