网纹甜瓜瓜汁对D-半乳糖衰老小鼠免疫功能的影响

目的观察网纹甜瓜瓜汁(简称瓜汁)对D-半乳糖衰老模型小鼠免疫功能的影响,以探讨其延缓衰老的免疫学机制。方法50只雄性昆明小鼠根据体重随机分为正常组、衰老模型组和三个不同稀释度的瓜汁组。衰老模型组和三个不同稀释度的瓜汁组小鼠皮下注射D-半乳糖200mg/kg·d,正常组注射等量的生理盐水;三个不同稀释度的瓜汁组分别灌胃4倍稀释的瓜汁溶液、2倍稀释的瓜汁溶液和瓜汁原液0.5ml,每天2次,正常组和衰老模型组灌胃等量的蒸馏水。连续4w,随后取胸腺、脾脏称重,并取血,分离出血清测定免疫球蛋白和补体含量。结果衰老模型组小鼠的胸腺指数比正常组显著降低,给予瓜汁后,胸腺指数与模型组相比较显示剂量相关性的升高,其中原液组有显著性差异。衰老模型组血清IgG、IgM、C3和C4显著低于正常组水平;给予瓜汁各组的血清IgG、IgM、C3和C4含量均较模型组有所升高,其中2倍稀释组和原液组的IgG、C3、C4含量显著升高。各组脾脏指数和血清IgA的结果也显示类似趋势。结论瓜汁能够减弱或对抗衰老过程中免疫器官的退行性改变,增强机体细胞和体液免疫功能,从而有助于延缓机体的衰老进程。

EFFECT OF MUSKMELON JUICE ON IMMUNE FUNCTION OF AGING MICE INDUCED BY D-GALACTOSE

Objective To study the effect of muskmelon juice on immune function of aged mice induced by D-galactose. Method Fifty male Kunming mice were randomly divided into five groups: normal control, aged model and three muskmelon juice (quadruple-diluted, double-diluted and stock juice) groups. The mice in aged model and three muskmelon juice groups were injected subcutaneously with D-galactose at dose of 200 mg/kg·d , and the normal control with the normal saline of equal volume. The mice in three muskmelon juice groups were treated by intragastric administration twice daily with quadruple-diluted solution, double-diluted solution and stock liquid of muskmelon juice respectively, and the mice in normal control and aged model group with distilled water. Four weeks later, spleen and thymus were weighted, and the immunoglobulim and complement in serum were estimated. Results The thymus index of mice in aged model groop was much lower as compared with normal control. After administration with muskmelon juice, the thymus index was increased and the difference between stock juice and aged model group was significant. As compared with normal control, the serum IgG, IgM, C3, C4 contents of aged model were significantly lowered, but those in muskmelon juice group was increased. The IgG, C3, C4 contents of double-diluted and stock juice group were significantly higher than those of aged model. The spleen index and serum IgA contents fluctuated with same tendency. Conclusion Muskmelon juice can relieve the regression of immune organs in aging process and improve the immune function, suggesting to be effective in anti-aging process.