干扰素α-2b对HL-60细胞抑增殖与促凋亡的影响

为了研究干扰素α-2b （IFNα-2b）对HL-60细胞增殖与凋亡的影响，采用瑞氏染色观察HL-60细胞的形态变化．吖啶橙／溴化乙锭双染色观察细胞凋亡，通过CDl3测定观察细胞膜抗原表达及成熟分化，MTT实验观察增殖活性。结果显示：加入IFNα-2b 48小时后，HL-60细胞凋亡率升高，增殖活性下降，而且随着IFNα-2b浓度的升高，抑制作用更加明显。CD13表达逐渐下降，细胞形态逐渐向成熟转化。结论：IFNα-2b能促进HL-60细胞凋亡、抑制HL-60细胞增殖和促进分化成熟。

Influence of Interferon α-2b on Proliferation Inhibition and Apoptosis Induction in HL-60 Cells

To investigate the effects of interferon alpha-2b on proliferation and apoptosis in HL-60 cells, HL-60 cells were cultured in different concentrations of IFN alpha-2b. The morphologic changes were observed by Wright's and acridine orange (AO) and ethidium bromide (EB) staining respectively. Inhibition of proliferation was detected by MTT. Expression of CD13(+) was checked by indirect fluoroimmunoassay. The results showed that apoptosis rate of HL-60 cells assayed by the above-mentioned two methods was (51 +/- 2)% and (78 +/- 3)% respectively and OD(570) values of proliferation inhibited were 1.8 +/- 0.1 and 1.0 +/- 0.1 respectively when the concentrations of the IFN(alpha-2b) were 500 and 10,000 U/ml in culture for 48 hours. Morphology and count of CD13(+) cells were changed. CD13(+) cell expression rate was (62 +/- 2)% and (30 +/- 3)% respectively when the concentrations of the IFN(alpha-2b) were 500 and 10,000 U/ml in culture for 48 hours. It is concluded that IFN(alpha-2b) can enhance the apoptosis of HL-60 cells, inhibit their proliferation, promote their maturation and differentiation.