| 胃癌及癌前期病变粘膜芳香基酰胺酶同工酶的表达意义 |
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| 引用本文： | 毛振彪, 孟宪镛, 葛政举, 倪润洲, 施公胜.胃癌及癌前期病变粘膜芳香基酰胺酶同工酶的表达意义[J].世界华人消化杂志,1998,6(4):323-325. |
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| 作者姓名： | 毛振彪 孟宪镛 葛政举 倪润洲 施公胜 |
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| 作者单位： | 226001，江苏省南通市西寺路27号,南通医学院附属医院消化研究室. |
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| 摘 要： | 目的探讨胃癌及癌前期病变粘膜芳香基酰胺酶（ＡＡＤ）同工酶的表达规律，寻找癌前期病变及早期癌的标志物．方法采用本室建立的圆盘等电聚焦电泳（ＣＩＥＦ）分离１２２例各种胃病粘膜（胃癌４１例，浅表性胃炎４８例，萎缩性胃炎３０例，胃溃疡８例，其中伴肠化１９例，异型增生８例）中ＡＡＤ同工酶．以６０ｇ／Ｌ丙烯酰胺作支持递质，ｐＨ３５～５０和ｐＨ３５～１００两种载体两性电解质建立ｐＨ梯度，加匀浆液５０μｌ，阴极及阳极电极液分别用０２ｍｏｌ／ＬＮａＯＨ和０５ｍｏｌ／ＬＨ３ＰＯ４，恒压电泳（１０℃）１８ｈ后以亮氨酰β萘胺作为基质呈色．结果ＡＡＤ分为８条区带，从阳极到阴极依次命名为ＡＡＤⅠ～ＡＡＤⅧ，其中ＡＡＤⅣ主要见于胃癌（９０２％），不完全大肠型肠化（８６７％）和异型增生（７５０％）；而不伴有肠化或异型增生的良性胃病和正常胃中则未发现．ＡＡＤⅣ的阳性率与胃癌病理类型、细胞分化程度及临床分期无相关，且ＡＡＤⅣ等电点和胎盘型ＡＡＤ等电点相当．结论ＡＡＤⅣ是一种癌胚蛋白，即胃癌组织特异性同工酶，是癌前期病变及早期癌的标志物．
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| 关 键 词： | 胃肿瘤／酶学 腺癌／酶学 癌前状态／酶学 氨基酸萘基酰胺酶类／代谢 同工酶类／代谢 胃粘膜／酶学 |
Expression of arylamidase isoenzyme in gastric carcinoma and precancerous lesions |
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| MAO Zhen Biao,MENG Xian Yong,GE Zheng Ju,LI Run Zhou and SHI Gong Sheng Research Section of Digestive Diseases,Affiliated Hospital,Nantong Medical College,Nantong ,Jiangsu Province,China.Expression of arylamidase isoenzyme in gastric carcinoma and precancerous lesions[J].World Chinese Journal of Digestology,1998,6(4):323-325. |
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| Authors: | MAO Zhen Biao MENG Xian Yong GE Zheng Ju LI Run Zhou and SHI Gong Sheng Research Section of Digestive Diseases Affiliated Hospital Nantong Medical College Nantong Jiangsu Province China |
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| Institution: | MAO Zhen Biao,MENG Xian Yong,GE Zheng Ju,LI Run Zhou and SHI Gong Sheng Research Section of Digestive Diseases,Affiliated Hospital,Nantong Medical College,Nantong 226001,Jiangsu Province,China |
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| Abstract: | |
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| Keywords: | stomach neoplasms/enzymology adenocarcinoma/enzymology precancerous conditions/enzymology amino acid naphthylamidases/metabolism isoenzymes/metabolism gastric mucosa/enzymology |
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