新砷化合物对小鼠C26移植性肿瘤细胞体内生长抑制作用的研究

目的探讨新砷化合物(AsI3-3Tu)对C26移植性肿瘤细胞的抑制作用及其对肿瘤组织环氧合酶-2(COX-2)及微血管密度(MVD)的影响.方法将Balb/c小白鼠随机分为对照组、5-Fu组和AsI3-3Tu组,于接种C26移植性肿瘤细胞后第6天开始腹腔注射给药,隔天1次,共给药8次,观察抑瘤率,并采用免疫组织化学的方法研究AsI3-3Tu对肿瘤组织COX-2及MVD的影响.结果 AsI3-3Tu对C26移植性肿瘤有明显的抑制作用,抑瘤率为55.07%.免疫组化结果显示,AsI3-3Tu对肿瘤组织的COX-2表达有明显的抑制作用,同时MVD明显降低.结论 AsI3-3Tu对C26移植性肿瘤有抑制作用,其作用途径可能是通过抑制COX-2的表达而抑制肿瘤血管的形成,进而抑制肿瘤的生长.

Inhibitory effect of new arsenide (AsI3-3Tu) on the growth of the transplanted sarcoma C26 in vitro

Objective To probe the effects of new arsenide (AsI 3-3Tu) on transplanted sarcoma C26 and on the expression of COX-2 and MVD in tumor tissue. Methods Balb/c mice were injected with transplanted sarcoma C26 and were randomizedly divided into control group,5-Fu group and AsI 3-3Tu group.The inhibitory rate on C26 was caculated routinely.The expression of COX-2 and MVD was detected by immunohistochemistry. Results The growth of C26 was significantly inhibited by AsI 3-3Tu,with the inhibitory rate of 55.07%(P<0.05).The expression of COX-2 in the tumor tissue was also inhibited by AsI 3-3Tu.And accordingly the expression of MVD was also markedly inhibited by AsI 3-3Tu. Conclusion AsI 3-3Tu had inhibitory effects on C26,and it decreased the expression of COX-2 in tumor tissue.There was positive relation between the expression of COX-2 and angiogenesis related factor.