参蛤散对压力负荷性心衰大鼠血流动力学及RhoA、ROCK1的作用

目的探讨参蛤散对压力负荷性心力衰竭大鼠血流动力学、左心室肌RhoA、ROCK1表达的影响。方法以不完全缩窄腹主动脉8周大鼠作为压力负荷性心力衰竭大鼠模型，随机分为假手术组、模型组、参蛤散组和对照组。参蛤散组以参蛤散灌胃（1．89g／kg）16周，对照组以法舒地尔腹腔注射（5mg／kg）16周，假手术组和模型组以生理盐水灌胃，观察大鼠血流动力学、RhoA、ROCK1表达的影响。结果与假手术组相比，模型组左室内收缩压（I。VSP）、左室内压力最大上升速率（＋dp／dtmax）明显下降（P〈0．01），左室内压力最大下降速率（-dp／dtmax）和RhoA、ROCK1表达明显升高（P〈0．01）；与模型组比较，参蛤散组LVSP、＋dp／dtmax明显升高（P〈0．05或P〈0．01）、dp／dtmax明显下降，RhoA的mRNA和蛋白表达明显下降，ROCK1的mRNA表达下降、蛋白表达无统计学意义；与法舒地尔组比较，参蛤散组I．VSP有所降低，-dp／dtmax有所上升，RhoA、ROCKl表达有所升高。各组间比较，左室舒张末期压（LVEDP）无统计学意义。结论参蛤散能有效提高压力超负荷诱导的心力衰竭大鼠血流动力学，抑制RhoA、ROCK1的表达，能在-定程度上改善心力衰竭。

Effects of Shenge Powder on the Hemodynamics and the Expression of RhoA and ROCK1 in Rats with Pressure - overload Heart Failure

Objective To explore the effect of Shenge powder （SGP） on the hemodynamics and the expression of RhoA and ROCKI in left ventricle of pressure - overload heart failure rats. Methods The pressure - overload heart failure was induced by incompletely abdominal aortic constriction. The rats was randomly divided into 4 groups：Sham group, control group, fasudil group and SGP group. The rats in SGP group were orally administration of SGP （1.89g/kg） ,and rats in fasudil group were administrated with fasudil（5 mg/kg）. The rats were sacrificed to test the hemodynamics and the expression of RhoA and ROCK1 16 weeks later. Results Compared with sham group, the left ventricular systolic pressure （I.VSP）, ＋ dp/dt of control group were reduced obviously, with the elevation of --dp/dt and the expression of RhoA and ROCK1. Compared with control group,the LVSP, ＋dp/dtmax were greater in SGP group with the contrary in --dp/dt the expression of RhoA and the mRNA expression of ROCK1. Compared with fasudil group, the LVSP was attenuated in SGP group with the elevation in --dp/dt and the expression of RhoA and ROCK1. Conclusion SGP could significantlly ameliorate hemodynamics in heart failure rats and inhibit the expression of RhoA and ROCK1. SGP could reverse heart failure to a certain extent.