潜阳合剂对自发性高血压大鼠胸主动脉重构的影响

目的观察潜阳合剂（QYHJ）对自发性高血压大鼠（SHR）胸主动脉血管重构的影响。方法将12周龄SHR随机分为QYHJ高、中、低剂量以及福辛普利组、SHR对照组，每组8只；另以8只同周龄雄性wKY大鼠为正常对照组。QYHJ高、中、低剂量组给药剂量分别为22．94g／kg、11．47g／kg和5．74g／kg，福辛普利组给药剂量为0．9mg／kg，连续给药8周。SHR对照组和正常对照组给予等容积双蒸水。至20周龄时采集胸主动脉标本，分别行苏木素伊红染色和Masson染色，测量各组大鼠胸主动脉重构指标。结果SHR对照组胸主动脉血管外径（ED）、内径（LD）、中膜厚度（MT）、中膜厚度与内径比（MT／LD）、血管中膜横截面积（MCSA）、血管横截面积（CSA）、血管中膜横截面积与血管横截面积比（MCSA／csA）、血管中膜胶原容积分数（CVF）值均较正常对照组增加（P〈0．05或P〈0．001），发生明显肥厚性重构。QYHJ高剂量组大鼠胸主动脉ED、LD、MCSA、CSA值较SHR对照组减小（P〈0．05或P〈0．01）；MT值较SHR对照组有减小趋势，但差异无统计学意义；MT／LD、MCSA／csA值与sHR对照组差异无统计学意义。SHR对照组胸主动脉血管中膜cVF值均较wKY大鼠增加（P〈0．001）；OYHJ高、中剂量组大鼠胸主动脉血管中膜CVF均较SHR对照组降低（P〈0．05或P〈O．01）。结论QYHJ对sHR胸主动脉肥厚性重构具有一定改善作用。

Effects of Qianyang Heji Decoction on the Arterial Remodeling of the Thoracic Aorta in Spontaneously Hypertensive Rats

Objective To observe the effects of Qianyang Heji decoction （QYHJ）on the arterial remodeling of the thoracic aorta in spontaneously hypertensive rats（SHR）. Methods Forty 12 week - old male SHR were assigned to 5 groups randomly：SHR control group, SHR＋ Fosinopril, SHR-- QYHJ High, SHR＋ QYHJ Medium, SHR; QYHJ low. Each group consisted of 8 animals. 8 male WKY rats （12 week- old） were used as normal control group. SHR＋ QYHJ High, Medium and Low were given QYHJ contained crude drug of 22.94 g/kg,ll. 47 g/kg,5.74 g/kg respectively; SHR＋Fosinopril were given suspl of fosinopril of 0.9 mg/kg; SHR and WKY control group were given the distilled water of the same volume. Drugs were given for 8 weeks constantly. Thoracic aorta was harvested at 20 week- old. After hematoxylin and eosin staining, Masson＇ s trichrome staining, the values of arterial remodeling of the thoracic aorta of each group rats were measured. Results The values of ED,LD,MT,MT/LD,MCSA,CSA,MCSA/CSA of tho- racic aorta of SHR were increased compared with that of WKY （P;0.05 or P;0. 001）. Hypertrophic remodeling was developed on the thoracic aorta of SHR. The values of ED,LD,MCSA,CSA of SHR＋QYHJ High were reduced compared with that of SHR （P〈0.05 or P〈0.01）. The values of MT of SHR＋QYHJ High were reduced compared with that of SHR, but there was no statistic sig- nificance. The values of MT/LD and MCSA/CSA of SHR were not significantly different from that of three groups of SHR＋QYHJ. The values of CVF of SHR were increased compared with that of WKY （P〈0. 001）. The values of CVF of SHR＋QYHJ High and Medium were reduced compared with that of SHR （P〈0.05 or P〈0.01）. Conclusion QYHJ could improve hypertrophic remode- ling of thoracic aorta in SHR.