OSTP增强紫杉醇诱导卵巢癌A2780细胞凋亡的作用

目的 研究卵巢癌特异性结合肽（OSTP）及联合紫杉醇对卵巢癌A2780细胞生长及凋亡的影响.方法 体外培养卵巢癌A2780细胞,随机分为4组：溶剂对照组（1640和0.1％ DMSO组）、OSTP组、紫杉醇组、OSTP联合紫杉醇组.采用四甲基偶氮唑盐（MTT法）检测OSTP对A2780细胞生长的影响;用Annexin-V-FITC和PI双染法标记A2780细胞,通过流式细胞仪检测细胞凋亡情况.结果 MTT法检测OSTP以不同浓度作用于卵巢癌A2780细胞时,可对该细胞的生长起到抑制作用,与对照组相比差异有统计学意义（P＜0.01）.应用流式细胞技术检测细胞凋亡发现：不同浓度的OSTP（20、40、80、160、320μmol/L）对A2780细胞的凋亡率分别7.88％、8.348％、8.727％、9.393％和10.68％,与对照组相比差异有统计学意义（P＜0.01）,提示OSTP可诱导细胞凋亡.OSTP和紫杉醇联合应用,在不同的预处理组（加OSTP80μ mol/L 0、3、6、12h）后再加紫杉醇10μmol/L作用48h,结果发现细胞凋亡率分别是39.40％、53.09％、48.18％和45.62％,均高于OSTP和紫杉醇单独用药及两者的凋亡率相加值,且差异有统计学意义（P ＜0.001）.提示OSTP能增强紫杉醇诱导的卵巢癌A2780细胞凋亡作用.结论 OSTP对卵巢癌A2780细胞有生长抑制及凋亡作用,尤其是OSTP能增强紫杉醇诱导卵巢癌A2780细胞凋亡的作用,对于探讨和开发OSTP作为靶向化疗增敏剂治疗卵巢癌有良好的应用前景.

OSTP Enhances Paclitaxel-induced Apoptosis in the Ovarian Cancer A2780 Cell

Objective We have firstly screened and identified the ovarian cancer specific targeting peptide （OSTP） in the preliminary work.In this study,we would investigate further the effect of OSTP combined with paclitaxel on the growth and apoptosis of ovarian cancer A2780 cells.Methods Ovarian cancer A2780 cells were cultured in vitro and then randomly divided into four groups：control group （included 1640 and 0.1％ DMSO） ; OSTP group ; paclitaxel group ; OSTP combined with paclitaxel group.MTT-assay was used to measure the inhibition ability of OSTP in A2780 cells proliferation.Annexin-V-FITC and PI double staining were used to label A2780 cells and FCM was used to detect the cell apoptosis.Results We detected that different concentrations of OSTP had an inhibited effect on the ovarian cancer A2780 cell by MTT-assay,and compared with the control group,the difference was statistically significant （P ＜0.01）.The results of FCM showed that the apoptosis rates of different concentrations of OSTP （20,40,80,160,320μmol/L） on A2780 cell were 7.88％,8.348％,8.727％,9.393％ and 10.68％,and compared with the control group,the difference was statistically significant （P ＜ 0.01）.It suggested us that OSTP could induce cell apoptosis,but the effect of apoptosis was not very strong.In different pretreatment groups （add OSTP 80μmol/L 0h,3h,6h,12h） plus paclitaxel 10μmol/L,the apoptosis rates were respectively 39.40％,53.09％,48.18％ and 45.62％.They were higher than OSTP and paclitaxel monotherapy and even their added value,and the difference was statistically significant （P ＜ 0.001）.It was suggested that OSTP could enhance paclitaxel-induced apoptosis in the ovarian cancer A2780 cell.Conclusion OSTP had an effect of growth inhibition and apoptosis on ovarian cancer A2780 cells,especially OSTP could enhance paclitaxel-induced apoptosis in the ovarian cancer A2780 cell.For the study and development of OSTP,it has a good application prospect as a targeting chemotherapy sensitizing agent for the treatment of ovarian cancer.