间充质干细胞复合同种异体脱钙骨的形态学观察

目的：探讨以间质干细胞（MSC）与同种异体脱钙骨复合培养法构建组织工程化生物衍生骨的可行性。方法：预先制备同种异体脱钙骨，取健康成人骨髓，用单核细胞分离液分离MSC，间充质干细胞基础培养基原代和传代培养。倒置显微镜下观察细胞生物学特性，用FITC标记的抗CD105对第3例细胞进行流式细胞鉴定，并与同种异体脱钙骨复合培养1周后行扫描电镜观察。结果：原代及传代培养的MSC增殖迅速，第3代CD105阳性细胞占64．1％，复合同种异体脱钙骨培养1周后细胞生长状态良好，增殖迅速，在材料表面形成细胞层。结论：未诱导的MSC是骨组织工程适宜的种子细胞，与同种异体脱钙骨复合可作为骨组织工程的载体。

Morphological observation of mesenchymal stem cells cultured with allogenic decalcified bone matrix]

OBJECTIVE: To investigate the feasibility of using decalcified bone matrix loaded with mesenchymal stem cells (MSCs) derived from adult human bone marrow as scaffolds for bone tissue engineering. METHODS: Allogenic decalcified bone matrix was prepared. MSCs were isolated from adult human bone marrow with monocyte-separating medium and then cultured in basic medium of mesenchymal stem cells (MSC). The third generation of the cultured cells were identified by FITC-antiCD105, seeded onto allogenic decalcified bone matrix followed by observation under scanning electron microscope and invert microscope a week later. RESULTS: Rapid proliferation of both primary and subcultured MSCs was observed, and CD105-positive cells reached 64.1% among the third generation of the cells. MSCs also grew well and proliferated rapidly after being cultured with decalcified bone matrix. CONCLUSION: Naive MSCs are ideal seeding cells for bone tissue engineering, and the allogenic decalcified bone matrix loaded with MSCs may be used as a good scaffold.