亚硒酸钠对微波致视网膜神经节细胞损伤的防护作用

目的 观察亚硒酸钠(sodium selenite,Na₂SeO₃)对微波致体外培养的视网膜神经节细胞损伤的防护作用。 方法 体外培养视网膜神经节细胞，按加入培养液Na₂SeO₃浓度分为4组，其中1组为对照组,30 mW/cm² 微波辐射1 h,测定细胞内的脂质过氧化指标及硒浓度，末端脱氧核甘酸转移酶介导的生物素化脱氧尿甘三磷酸末端标记法(TdT-mediated dUTP nick end labeling,TUNEL)检测细胞凋亡。 结果 1×10⁷mol/L Na₂SeO₃即可使细胞内超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutatione peroxidase,GSH-Px)活性较未加硒组明显升高(P<0.05),丙二醛(malondialdehyde,MDA)含量显著下降(P<0.05),凋亡细胞数量减少,1×10⁶ mol/L组和1×10⁵ mol/L组在抗氧化能力方面优于1×10⁷ mol/L组,但这两组间无显著差异。 结论 Na₂SeO₃对微波致视网膜神经节细胞的损伤具有一定的保护作用。 (中华眼底病杂志,2000,16:97-99)

The protective effects of Na2SeO3 on the damage of retinal neuron induced by microwave

ObjectiveTo observe the protective effects of Na₂SeO₃ on the damage of retinal neuron induced by microwave. MethodsCultured fluids of retinal neuron were divided into 4 groups,including 1 group of control, according to the concentration of Na₂SeO₃ in cultured fluid and then exposed to 30 mW/cm² microwave for 1 hour.The targets of lipid peroxidation and the concentration of selenium in cells were measured.Apoptosis detection was taken by TUNEL detection kit. ResultsThe activity of SOD and GSH-Px rised,meanwhile the content of MDA and the amount of apoptosis cells decreased in 1×10⁷ mol/L group compared with the group without Na₂SeO₃.The other groups was superior in antioxdant capacity to 1×10⁷ mol/L group. ConclusionNa₂SeO₃ might be possessed of the effect of protecting the damage of retinal neuron induced by microwave.(Chin J Ocul Fundus Dis,2000,16:97-99)