不同方式骨髓移植对辐射小鼠GVHD反应影响的研究

目的 探讨不同方式骨髓移植的移植物抗宿主反应。方法 受体C₅₇BL/6小鼠以⁶⁰Co辐射,经由尾静脉注入供体细胞。观察GVHD一般反应及病理学改变;通过解剖镜下计数脾结节、半固体琼脂法检测CFU-GM及常规计数外周WBC变化进行造血功能测定。G显带技术测定不同移植方式的嵌合体。结果 同基因与混合半相合组30 d存活率高于其他两组,异基因组GVHD反应较强,半相合组次之,混合半相合组最弱,移植13 d时同基因组与混合半相合组脾结节无明显差别,但均高于异基因或半相合组,第18天时混合半相合嵌合体与半相合组无明显差异,第30天时混合半相合组嵌合体较高。异基因、半相合、混合半相合及同基因组的体内混合淋巴细胞反应程度,依次从强到弱。除异基因组的外周血WBC及骨髓CFU-GM较低外,其余各组无明显差别。结论 半相合与混合半相和骨髓移植可以减少GVHD反应。

Comparison of GVHD in Different Sorts of Bone Marrow Transplantation

Objective To study GVHD in different sorts of BMT.Methods C 57BL/6 recipient mice irradiated with 60Co were transfused with donor cells via the tail vein.The general manifestation and pathological changes of GVHD were observed.The hematopoietic function was evaluated with the spleen node count under microscope,semi-solid agar CFU-GM assay and peripheral blood WBC count.The C banding technique was used to detect the chimeric rate in different sorts of BMT.Results The survival rate on day 30 in syngeneic group or mixed half-matched group was higher than two other groups.The GVHD reaction was strong in allogeneic group,to a less extent in half-matched group,and the least in mixed half-matched group.Spleen node count on day 13 showed no significant difference between the syngeneic and the mixed half-matched group although both groups had larger numbers than the allogeneic or the half-matched group.The chimeric rate in mixed half-matched group was not different from the half-matched group on day 18,while it was higher in the mixed half-matched group on day 30.The extent of mixed lymphocyte reaction in vivo was in descending sequence in allogeneic,half-matched,mixed half-matched and syngeneic BMT group.The peripheral blood WBC count and bone marrow CFU-GM assay showed no significant difference among other groups except that they are lower in the allogeneic group.Conclusion Half-matched and mixed half-matched BMT could alleviate the GVHD reaction.