葡多酚对辐射后淋巴细胞凋亡的影响

①目的探讨葡多酚(GPC)对人淋巴细胞辐射损伤后凋亡的影响.②方法选取7名健康女性的外周血,分别加入100、10 mg/L GPC溶液(高、低剂量GPC组),阳性和阴性对照组加入无菌蒸馏水,前3组用直线加速器行X线一次性照射.分别在培养至24和48 h检测淋巴细胞凋亡率.③结果与阳性对照组比较,高剂量GPC组淋巴细胞凋亡率明显降低(F＝28.031、18.998,q＝5.820、5.656,P＜0.01).④结论葡多酚可降低X线对淋巴细胞损伤后凋亡的程度,对淋巴细胞辐射损伤有良好防护作用.

THE EFFECT OF GRAPE PROCYANIDINS ON APOPTOSIS OF LYMPHOCYTES AFTER RADIATION

Objective To study the effect of grape procyanidins(GPC) on apoptosis of human lymphocytes after radiation. Methods The peripheral blood samples were taken from 7 healthy young women, 100 mg/L and 10 mg/L GPC solution was added to High-dose and Low-dose GPC groups, respectively; for Positive and Negative groups, distilled water was added to. They were irradiated with X-ray with liner accelerator, with the exception of negative group. After culture for 24 and 48 hours, apoptosis rate of the lymphocyte was measured. Results Compared with Positive group , the apoptosis rate of High-dose GPC group was much lower(F=28.031,18.998;q=5.820,5.656;P<0.01). Conclusion GPC has a satisfactory protective effect on radiation injury of the lymphocyte, it can reduce the apoptosis rate caused by X-ray.