多视场多参数形态定量分析模块在神经研究领域中的应用

缺血性神经元损伤的性质是神经研究关注的热点。但目前用于区分凋亡与坏死细胞的ＴＵＮＥＬ技术，较易受主观因素的影响。本实验和图像分析技术对缺血动物脑的ＴＵＮＥＬ染色切片进行多场多参数形态定量分析，即细胞形态这亘参数（核面积、周长、直径、形状因子）和含 密度（平均光密度、积分光密度）。本实验结果显示图像分析能提高ＴＵＮＥＬ鉴别凋亡与坏死的灵敏度和精确性，并提供了一种简便快捷的形态定量方法，是在神经研究中

Performance of image analysis system as a morphological quantitative method

It pays close attention to the mechanis ms of theischemic neuronal dama ge in the field of neuroscience. TUNEL method which is used todiscriminate apop totic cell death from necrotic one is easily influenced by many factorselse. Th e present study measured neuronal damage on TUNEL slices by image cytometry after suffered with focal cerebral ischemia in vivo. It included morphological quant itativeparameters (nucleus area, perimeter, diameter and form factor) and gray level. The dataindicated that image analysis system may raise the sensitivity a nd precision of TUNELmethod. As a good morphological quantitative method it sho uld be used to do someresearches on neuroscience.