A novel in vitro DNA packaging system demonstrating a direct role for the bacteriophage lambda FI gene product |
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| Authors: | A Davidson M Gold |
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| Affiliation: | Department of Medical Genetics, University of Toronto, Ontario, Canada. |
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| Abstract: | A new in vitro bacteriophage lambda DNA packaging system is described in which all the proteins necessary for head morphogenesis are supplied by extracts of plasmid-transformed cells. This assay is used to demonstrate that the lambda FI gene product (gpFI) is necessary for maximal packaging efficiency when proheads and terminase are present in limiting amounts. A 100- to 200-fold decrease in packaging is seen when gpFI is omitted. gpFI is shown to act at and/or after the stage in packaging where proheads bind to the DNA:terminase complex. |
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