程序性死亡受体1、程序性死亡配体1和神经生长因子在肝癌组织中的表达及意义

目的检测肝细胞癌组织中程序性死亡受体1(programmed death 1,PD-1)及其主要配体程序性死亡配体1(programmed death ligand-1,PD-L1)、神经生长因子(nerve growth factor,NGF)的表达与不同病理分级、肝癌临床分期、肝功能及AFP的关系。方法采用免疫组织化学法检测63例肝癌石蜡包埋组织标本中PD-1、PD-L1和NGF的表达量,16例正常供体肝脏石蜡包埋组织标本作为正常对照组。比较不同病理分级、不同肝癌临床分期、不同肝功能及不同AFP水平情况下肝癌组织中PD-1、PD-L1和NGF的表达差异。结果肝癌组织中PD-1、PD-L1和NGF阳性表达率高于正常对照组(P0.05),不同病理分级肝癌组织中PD-1、PD-L1及NGF的阳性表达率不同,以中分化肝癌最高(P0.05)。PD-1、PD-L1及NGF阳性表达率以肝癌巴塞罗那分期C期升高最为显著(P0.05)。不同肝功能Child分级患者肝癌组织中PD-1、PD-L1和NGF阳性表达率差异无统计学意义。肝癌组织PD-1、PD-L1、NGF阳性表达率在血清AFP水平≥400μg/L与400μg/L患者中差异有统计学意义,前者高于后者,而在年龄≥50岁与50岁患者中差异无统计学意义。结论肝癌患者肝癌组织PD-1、PD-L1和NGF阳性表达率明显升高。PD-1、PD-L1和NGF在肝癌组织中的表达可能在肝癌发生、发展及转移过程中可能发挥一定作用。

Expressions of programmed death 1, programmed death ligand-1 and nerve growth factor in liver cancer tissues and their significance

Objective To detect the protein expressions of programmed death 1 (PD-1), programmed death ligand-1 (PD-L1) and nerve growth factor (NGF) in hepatocellular carcinoma (HCC) tissue and investigate their relationships with different pathological classification, clinical liver cancer stages, liver function and AFP level.Methods Immunohistochemistry (IHC) was used to detect the protein expressions of PD-1, PD-L1 and NGF in the paraffin-embedded tissue specimens of 63 HCC patients. Paraffin-embedded liver tissue specimens from 16 healthy donors were used as the controls. The expressions of PD-1, PD-L1 and NGF in HCC tissues of different pathological classification, clinical liver cancer stages, liver function and AFP level were compared.Results The positive expression rates of PD-1, PD-L1 and NGF in HCC tissue specimens were significantly higher than that in the controls (P<0.05). The positive expression rates of PD-1, PD-L1 and NGF in HCC tissue specimens with different pathological classification varied, being the highest in moderately differentiated HCC tissues (P<0.05). The positive expression rates of PD-1, PD-L1 and NGF in HCC tissue specimens with Barcelona clinical liver cancer stage C increased (P<0.05). The differences in the positive expression rates of PD-1, PD-L1 and NGF in HCC tissue specimens with different Child-Pugh grades were not significant. The differences in the positive expression rates of PD-1, PD-L1 and NGF in HCC tissue specimens were significant between patients with AFP level≥400μg/L and those with AFP level<400μg/L, and the rates in the former were significantly higher than those in the latter. There was no significant difference in the positive expression rates of PD-1, PD-L1 and NGF in HCC tissue specimens between patients aged over and equal to 50 and those aged less than 50.Conclusions The positive expression rates of PD-1, PD-L1 and NGF in HCC tissues increase significantly. The expressions of PD-1, PD-L1 and NGF in HCC tissues may play a role in the occurrence, development and metastasis of HCC.