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| 骨碎补总黄酮对去卵巢大鼠组织蛋白酶K的影响 | |
| 引用本文： | 史晓林,李春雯,孙金谞,何伟涛,谢海宝. 骨碎补总黄酮对去卵巢大鼠组织蛋白酶K的影响[J]. 中华骨质疏松和骨矿盐疾病杂志, 2010, 3(4): 285-290. DOI: 10.3969/j.issn.1674-2591.2010.04.010 |
| 作者姓名： | 史晓林 李春雯 孙金谞 何伟涛 谢海宝 |
| 作者单位： | 1. 浙江中医药大学附属第二医院骨科,杭州,310005 2. 浙江中医药大学基础医学院,杭州,310053 3. 浙江医院老年医学科,杭州,310013 |
| 摘 要： | 目的观察骨碎补总黄酮对去卵巢大鼠骨质疏松模型组织蛋白酶K(Cathepsin K)的血清浓度和左胫骨干骺端基因表达的影响,探讨骨碎补总黄酮防治骨质疏松的机制。方法选取3月龄雌性SD大鼠72只,随机分为6组:高剂量骨碎补总黄酮组[治疗组1,12只大鼠,切除双侧卵巢,给药剂量0.216 g/(kg·d)];中剂量骨碎补总黄酮组[治疗组2,12只大鼠,切除双侧卵巢,给药剂量0.108 g/(kg·d)];低剂量骨碎补总黄酮组[治疗组3,12只大鼠,给药剂量0.054 g/(kg·d)];雌激素组(12只大鼠,切除双侧卵巢,给予结合雌激素片0.1 mg/kg);假手术组(12只大鼠,仅切除腹腔少量脂肪和软组织,以5 mL蒸馏水灌胃);正常组(12只大鼠,不做特殊处理,以5 mL蒸馏水灌胃)。各组均在去卵巢造模成功后开始灌胃,并于造模成功后即刻、3个月、6个月每组处死4只大鼠,无菌条件下抽取静脉血,检测血清Cathepsin K浓度。取左胫骨近端干骺端标本,荧光定量PCR测定Cathepsin K mRNA表达量,用SPSS13.0软件进行数据分析。结果各治疗组、正常组与雌激素组大鼠血清Cathepsin K浓度间差异均有统计学意义(P0.05)。各治疗组与雌激素组左胫骨干骺端Cathepsin K mRNA表达量相比有统计学意义(P0.05)。各治疗组、假手术组与正常组左胫骨干骺端Cathepsin K mRNA表达量相比均有统计学意义(P0.01)。结论骨碎补总黄酮抑制去卵巢大鼠血清Cathepsin K浓度、降低左胫骨干骺端Cathepsin K mRNA表达量的作用,可能是其治疗骨质疏松症的机制之一。 |
| 关 键 词： | 骨碎补总黄酮 去卵巢大鼠 组织蛋白酶K |
The effect of drynaria total flavonoids on expression of Cathepsin K in ovariectomized rats |
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| Affiliation: | SHI Xiao-lin LI Chun-wen SUN Jin-xu 1. Department of Orthepadics, Second Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou 310005; 2. Zhejiang Chinese Medical University College of Basic Medical Seienee, Hangzhou 310053; 3. Department of Geriatrics , Zhejiang Hospital, Hangzhou 310013, China |
| Abstract: | Objective To investigate the effect of drynaria total flavonoids on serum concentration of Cathepsin K and its expression in left tibial metaphysis in ovariectomized rats, and investigate the mechamism of of osteoporosis prevention by total flavonoids. Methods 3 month old female SD rats were randomly divided into 6 groups： high-dose total flavonoids group [treatment group 1 , 12 rats were ovariectomized, dose 0. 216 g/ （ kg· d） ] ; middle-dose total flavonoids group [ treatment group ：2 , 12 rats were ovariectomized, dose 0. 108 g/ （kg·d） ] ; low-dose total flavonoids group [ treatment group 3 , 12 rats were ovariectomized, dose 0. 054 g/ （ kg ·d） ] ; estrogen group （ 12 rats were ovariectomized, conjugated estrogen tablets dose 0. 1 mg/kg） ; sham operation group （ 12 rats, only remove a small amount of |
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