经胎盘暴露壬基酚对仔鼠神经行为发育的影响

目的 探讨母体壬基酚(NP)暴露对子代大鼠神经行为发育的影响.方法 将确定受孕的SD大鼠随机分为对照组及50、100、200 mg/kg NP组,每组6或7只,妊娠第9-15 d灌胃NP.观察NP对仔鼠生理发育、神经行为发育的影响;于出生后1、7、14、21、28 d测体重,脱椎取大脑海马组织行HE染色,光学显微镜下观察其病理变化.结果 200 mg/kg NP剂量组仔鼠早期生理发育指标张耳、长毛、出牙、开眼时间分别为(4.5±0.8)、(5.2±0.8)、(12.7±1.4)、(16.0±7.7)d,较对照组[(3.6±0.5)、(3.6±0.5)、(11.1±1.1)、(12.7±1.3)d]明显延长,差异有统计学意义(p<0.05),200mg/kgNP剂量组仔鼠的断崖回避、平面翻正、空中翻正、负趋地性、听觉惊愕和视觉定向发育时间分别为(9.8±1.0)、(6.5±0.8)、(11.3±0.5)、(9.2±o.8)、(11.2±1.0)、(20.2±1.0)d,比对照组[(8.0±0.6)、(5.1±0.4)、(8.3±0.5)、(9.2±0.8)、(9.3±0.5)、(9.3±0.5)d]明显延长,差异有统计学意义(P<0.05或P<0.01).100 mg/kg NP剂量组仔鼠平面翻正、断崖回避、视觉定向时间均比对照组明显延长,差异有统计学意K(P<0.05或P<0.01).病理学检查可见100、200 mg/kg NP组海马组织充血水肿.结论 NP宫内暴露可影响仔鼠神经行为的发育.

Effects of nonylphenol exposure via placenta on early nervous reflex and locomotives of offspring in rat

Objective To explore the influence of nonylphenol(NP)on the neural behavioral devel-opment of filial generation rats exposed via placenta.Methods On the first day of the pregnancy.the SD rats were divided into four groups,and orally administered with NP at doses of 0,50,100 and 200 mg/kg on gesta-tional day 9-15 respectively.The offspring rats of each groups were examined to obsenre the impact of NP on the early physiological,neurobehavioral development.The changes of filial generation body weight(from gen-eration day 1 to 28)were measured.Brain tissues were stained with Hematoxylin-eosin and Congo red to ob-serve with optical microscope.Results In contrast to the control group,the early physiological markers(pin-na detachment,hairgrowth.tooth growth and eye opening)and the early neurobehavioral development indices ( surface nghring,air fighting,acoustic startle and visual placing)were significantly delayed in the groups ofNP 200mg/kg dose(P<0.05).The developing time of physiological markers decreased from(4.5±0.8.5.2±0.8,12.7±1.4,16.0±1.7)d to(3.6±0.5.3.6±0.5,11.1±1.1,12.7±1.3)d while neurobehavioral developing time decreasedfrom(6.5±0.8,11.3±0.5,11.2±1.0,20.2±1.0)d to(5.1±0.4,8.3±0.5,9.3±0.5.9.3±0.5)d(P<0.05or P<0.01).The body weights of filial generation rats were decreased obviously from lst day to 28th day.Histopathological examination displayed that hippocampal neurons had congestion and oedema in the group of 100,200 mg/ks dose.Conclusion Exposures to NP during gestation might impair the neurobehavioral devel-opment of F1 rats significantly.