雄性激素对小鼠骨髓巨噬细胞杜氏利什曼原虫感染水平的影响

目的：研究雄性激素对杜氏利什曼原虫感染的C57BL／6J小鼠骨髓巨噬细胞（BMMs）水平的影响。方法：用雄性激素处理小鼠3wk后，收集BMMs，培养5d后，按BMMs前鞭毛体＝110的比例，接种杜氏利什曼原虫，用吉姬氏染色法观察不同时相的感染水平。结果：与植物油处理的对照组相比，雄性激素处理的小鼠BMMs对前鞭毛体较为易感，并随着感染时间的延长其感染水平增高（感染后3h，P＜0．05；24h，48h和72h，P＜0．01）。结论：雄性激素可增加杜氏利什曼原虫对BMMs 的感染水平, 这可能与雄性激素诱导的免疫抑制作用有关。

EFFECT OF TESTOSTERONE ON LEISHMANIA DONOVANI INFECTION LEVELS OF MURINE BONE MARROW DERIVED MACROPHAGES

AIM: To investigate the effect of the male sex hormone, testosterone (Te), on Leishmania donovani infection levels of bone marrow derived macrophages(BMMs) from female mice of strain C57BL/6J. METHODS: After three weeks of Te treatment, the BMMs were isolated, challenged with L.donovani at a ratio of 10 to 1 promastigotes per macrophage, and the infection levels of different time points were monitored by Giemsa staining. RESULTS: BMMs from Te treated mice had a significantly increased initial uptake(3 h post infection, P<0 05) of promastigotes and carried heavier infection levels at all time points(24 h, 48 h, 72 h post infection, P<0 01), compared with those from oil treated controls. CONCLUSION: Te can increase L.donovani infection levels of BMMs, being possibly related to Te induced immunosuppression.