| SLT温控酶标仪微量快速测定ALT在血站初筛中的应用 |
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| 引用本文： | 周祺芳.SLT温控酶标仪微量快速测定ALT在血站初筛中的应用[J].临床输血与检验,2000,2(3):20-21. |
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| 作者姓名： | 周祺芳 |
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| 作者单位： | 安徽省宣州市中心血站 242000 |
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| 摘 要： | 目的 评价SLT温控酶标仪检测ALT在血站初筛中的应用。方法 选择17998份血清标本，比较SLT温控酶标仪和终点比色法检测ALT活性的结果。结果 两法符合率99．8％。SLT酶标仪测量ALT的批内和批间变异分别是2．99％和3．30％，平均回收率96，7％。该法与常规连续监测法显著相关，回归方程Y=0，999X 1，05，r^2=0．993。结论 本法具有快速、准确、稳度等优点，适合在血站初筛中应用。
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| 关 键 词： | 初筛 血站 ALT活性 SLT 酶标仪 血清标本 批间变异 快速 准确 |
Application of micro-rapid method for measuring ALT activity in donor's screening with SLT temperature control reader |
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| Zhou Qifang.Application of micro-rapid method for measuring ALT activity in donor''''s screening with SLT temperature control reader[J].Journal of Clinical Transfusion and Laboratory Medicine,2000,2(3):20-21. |
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| Authors: | Zhou Qifang |
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| Institution: | Zhou Qifang. The Central Blood Bank,Xuanzhou 242000 |
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| Abstract: | Objective To evaluate the application in measuring ALT activity with SLT temperature control reader. Method 17 998 sera were selected and kinetic assay method was applied to detect the ALT activity compared with end-point method. Results Kinetic assay provided the same conclusions as given by endpoint method to an extent of 99. 8% in analysing 17 998 samples. The within-run CV and between-run CV were 2. 99% and 3. 30% respectively. The average rate of recovery was 96. 7%, and the correlation between kinetic assay and continuous surveillance was significant: Y = 0. 999 X 1. 05.r2 = 0. 993 . Conclusion It suggest that the method is rapid,accuratate,stable for donor's screening. |
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| Keywords: | SLT temperature control reader ALT |
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