| 细胞系中支原体检测及分型 |
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| 引用本文： | 戴悦,吕允威,张春凤,曹邦伟,柴庆波,马明信,柯杨. 细胞系中支原体检测及分型[J]. 北京大学学报(医学版), 2005, 37(2): 207-210 |
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| 作者姓名： | 戴悦 吕允威 张春凤 曹邦伟 柴庆波 马明信 柯杨 |
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| 作者单位： | 北京大学临床肿瘤学院,北京市肿瘤防治研究所遗传室,北京,100034;北京大学肿瘤研究中心;北京大学第一医院输血科 |
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| 基金项目： | 北京大学校科研和教改项目 , 国家科技攻关项目 |
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| 摘 要： | 目的:对细胞系中支原体的感染情况进行检测并分型.方法:利用支原体16S～23S保守区域扩增来检出细胞系中支原体感染率,并利用16S～23S保守区间的间隔区(spacer)长度不同进行巢式PCR扩增,结合测序达到分型的目的.结果:在检测的22株细胞系中,有77.3%(17株)的细胞系有支原体感染,其中5株有2种或2种以上支原体感染,在分布上以M.fermentans和M.hyorhinis支原体多见.结论:支原体在细胞系中的高感染率提示,在以细胞系作为模型的研究中,对支原体感染所造成的影响应有充分认识.检测及分型技术的建立为进一步研究不同型别支原体的致病力提供了可能.
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| 关 键 词： | 支原体属 细胞培养 聚合酶链反应 |
| 文章编号： | 1671-167X(2005)02-0207-04 |
| 修稿时间： | 2004-10-08 |
Detection and classification of Mycoplasma in cell lines |
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| DAI Yue,LV Yun-wei,ZHANG Chun-feng,CAO Bang-wei,CHAI Qing-bo,MA Ming-xin,KE Yang. Detection and classification of Mycoplasma in cell lines[J]. Journal of Peking University. Health sciences, 2005, 37(2): 207-210 |
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| Authors: | DAI Yue LV Yun-wei ZHANG Chun-feng CAO Bang-wei CHAI Qing-bo MA Ming-xin KE Yang |
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| Affiliation: | Department of Genetic, Peking University School of Oncology, Beijing 100034, China. |
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| Abstract: | OBJECTIVE: To detect the rate of Mycoplasma infection in cell lines and further determine its types. METHODS: We performed nest PCR amplification of Mycoplasma's conserved regions (16S-23S) and sequenced the spacer with different length between conserved regions. RESULTS: Within the tested 22 cell lines, 17 (77.3%) showed Mycoplasma infections, of which 5 had two or more types of Mycoplasma. M. fermentans and hyorhinis were more frequently detected within the types of infected Mycoplasma. CONCLUSION: The high rate of Mycoplasma infection in cell lines makes it necessary for researchers to pay more attention to its influence on research data when using cell lines as models. Establishment of detection and classifying techniques make it possible to further study the pathogenesis of different types of Mycoplasma. |
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| Keywords: | Mycoplasma Cell culture Polymerase chain reaction |
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