Ghrelin-induced growth hormone release from goldfish pituitary cells is nitric oxide dependent |
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Authors: | Caleb L. GreyJohn P. Chang |
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Affiliation: | Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E9 |
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Abstract: | Ghrelin (GRLN) is an important neuroendocrine regulator of growth hormone (GH) release in vertebrates. Previous studies show goldfish (g)GRLN19-induced GH from the goldfish pituitary involves voltage sensitive Ca2+ channels, increases in intracellular Ca2+ and the PKC signalling pathway. We set out to examine the role of the nitric oxide (NO) pathway in gGLRN19-induced GH release from primary cultures of goldfish pituitary cells using pharmacological regulators in cell column perifusion systems. The NO scavenger PTIO abolished gGRLN19-induced GH release and co-treatment with the NO donor SNP and GRLN did not produce additive GH release responses. Nitric oxide synthase (NOS) inhibitors 1400 W and 7-Ni abolished GRLN-induced GH release while treatment with another NOS inhibitor, AGH, had no significant effect. Taken together, these results demonstrate that the NOS/NO is an integral component of gGRLN19-induced signalling within the goldfish pituitary cells, and given the relative specificity of AGH for inducible NOS and endothelial NOS isoforms, suggests that neuronal NOS is the likely NOS isoform utilized in goldfish somatotropes by this physiological regulator. |
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Keywords: | Goldfish ghrelin Nitric oxide synthase isoform-selective inhibitors Signal transduction Primary goldfish pituitary cell culture NO scavenging |
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