23-羟基白桦酸诱导LoVo细胞凋亡与细胞内活性氧的关系

目的： 研究23-羟基白桦酸诱导人结肠癌细胞株LoVo细胞凋亡过程中活性氧（ROS）的变化及其作用机制。方法：光学显微镜法观察23-羟基白桦酸作用LoVo细胞后细胞形态学改变，并采用流式细胞仪检测细胞凋亡及细胞内ROS水平。结果：分别以浓度为25、50、100、200 μmol/L 23-羟基白桦酸作用LoVo细胞48h后，在光学显微镜下观察到明显的凋亡细胞形态学改变；经流式细胞仪检测，细胞凋亡率分别为(7.17±2.31)%、(15.60±4.02)%、(32.47±5.25)%及(52.71±5.93)%，呈现一定的浓度依赖性关系。23-羟基白桦酸可使 LoVo 细胞内ROS的水平明显高于空白对照组。细胞内ROS含量（MFI，平均荧光强度）分别为2.83±0.80、5.97±1.72、12.53±2.57及16.73±4.58。其中，浓度为100和200 μmol/L 23-羟基白桦酸的作用较明显，与对照组(2.13±0.32)比有显著差异（P<0.05）。结论：23-羟基白桦酸具有明显的诱导LoVo细胞凋亡作用，其作用机制可能与23-羟基白桦酸引起细胞内ROS水平增加有关。

Role of reactive oxygen species in 23-hydroxybetulinic acid-induced apoptosis in LoVo cells

AIM: To investigate the changes of reactive oxygen species (ROS) in apoptosis of LoVo cells induced by 23-hydroxybetulinic acid.METHODS: LoVo cells were treated with 23-hydroxybetulinic acid. The apoptotic morphological change was observed under the light microscope. Intracellular ROS production and the rate of apoptosis were detected by flow cytometry.RESULTS: LoVo cells improved apoptotic morphological changes treated with 23-hydroxybetulinic acid for 48 h. At concentrations of 25, 50, 100, 200 μmol/L of 23-hydroxybetulinic acid, the apoptotic rates of LoVo cells were (7.17±2.31)%, (15.60±4.02)%, (32.47±5.25)% and (52.71±5.93)%, respectively. The results indicated a certain concentration-dependent relationship. 23-hydroxybetulinic acid caused an increase in the ROS production, and the ROS levels were 2.83±0.80, 5.97±1.72, 12.53±2.57 and 16.73±4.58. Compared with the control group (2.13±0.32), the increase in ROS production in LoVo cells at the concentration of 100, 200 μmol/L of 23-hydroxybetulinic acid treatment was significant (P<0.05). CONCLUSION: 23-hydroxybetulinic acid induces LoVo cell apoptosis. The production of ROS may play a crucial role in the process of the LoVo cell apoptosis.