两种LsAB染色标记方法及其抗原修复方法的比较

目的 比较两种标记生物素链亲和素法(LsAB)，并探讨抗原修复的最佳方法。方法 采用碱性磷酸酶(AP)LsAB法和辣根过氧化物酶(HRP)LsAB法染色肺癌合并慢性阻塞性肺疾病(COPD)患者肺组织石蜡切片中类胰蛋白酶(tryptase)的抗肥大细胞类胰蛋白酶抗体(AA1，小鼠抗人)，并比较染色效果；用高压、胰蛋白酶和微波三种方法进行抗原修复，比较修复效果。结果 AP-LsAB法染色比HRP-LsAB法背景清晰、颜色对比鲜明；高压修复效果优于胰蛋白酶和微波修复。结论 在肺组织免疫组化染色中推荐使用AP-LsAB法，并采用高压修复抗原。

Comparing Two Kinds of Labeled Streptavidin Biotin Methods and Selecting the Best Antigen Retrieval Method

Objective To compare two kinds of labeled streptavidin biotin(LsAB) methods and provide the optimal method of antigen retrieval. Methods The alkaline phosphatase(AP) LsAB method and the horseradish peroxidase(HRP) LsAB method were used to stain anti-tryptase in paraffin embedding tissue of patients with chronic obstructive pulmonary disease(COPD) and their staining effects were compared. The antigens were repaired by high pressure cooking,tryptin digestion and microwave,and the repairing effects were compared. Results The backgrounds of stained sections were more distinct and the color distinction of nucleus and positive signal were brighter by AP-LsAB, compared with those by HRP-LsAB; the repairing effect of high pressure cooking was better than the repairing effect of tryptin digestion and than that of microwave. Conclusion AP-LsAB and high pressure cooking retrieval were recommended for use in immunohistochemical staining of pulmonary inflammatory tissue.