| Inhibitory Effect of Pulmonary Carcinoma by Adenovirus-Mediated CD/UPRT Gene |
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| 作者姓名： | 黄畦 陈大瑜 付向宁 祖育昆 |
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| 作者单位： | Department of Thoracic and Cardiac Surgery Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Thoracic and Cardiac Surgery，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Thoracic and Cardiac Surgery，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Thoracic and Cardiac Surgery，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China |
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| 摘 要： | The cell killing effects and bystander effects of double suicide gene on pulmonary carcinoma cells were explored. Lung adenocarcinoma cells (A549) were transfected with different liters of adenovirus vector and followed with different concentrations of 5-FC after a recombinant adenovirus vector carrying CD/UPRT gene (Ad-CD/UPRT) was constructed. The cell viability was measured by MTT assay 4 days later. The cell viability was dropped to 30.57 %-8.62 % after 10 MOI of Ad-CD/UPRT transfected and 5-FC (10-1000μg/mL) administration. Furthermore, Ad-CD/UPRT-infected A549 cells showed a profound neighbor cell killing effect in the same methods. These results suggested that Ad-CD/UPRT/5-FC system can effectively suppress growth of lung adenocarcinoma cells, which may provide a novel and powerful candidate for lung cancer gene therapy strategies.
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| 关 键 词： | 肺癌 基因治疗 病理机制 临床 |
| 收稿时间： | 2006-01-16 |
Inhibitory effect of pulmonary carcinoma by adenovirus-mediated CD/UPRT gene |
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| Qi Huang,Dayu Chen,Xiangning Fu,Yukun Zu.Inhibitory Effect of Pulmonary Carcinoma by Adenovirus-Mediated CD/UPRT Gene[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006,26(5):591-593. |
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| Authors: | Qi Huang Dayu Chen Xiangning Fu Yukun Zu |
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| Institution: | Department of Thoracic and Cardiac Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | The cell killing effects and bystander effects of double suicide gene on pulmonary carcinoma cells were explored. Lung adenocarcinoma cells (A549) were transfected with different titers of adenovirus vector and followed with different concentrations of 5-FC after a recombinant adenovirus vector carrying CD/UPRT gene (Ad-CD/UPRT) was constructed. The cell viability was measured by MTT assay 4 days later. The cell viability was dropped to 30.57 %-8.62 % after 10 MOI of Ad-CD/UPRT transfected and 5-FC (10-1000 microg/mL) administration. Furthermore, Ad-CD/UPRT-infected A549 cells showed a profound neighbor cell killing effect in the same methods. These results suggested that Ad-CD/UPRT/5-FC system can effectively suppress growth of lung adenocarcinoma cells, which may provide a novel and powerful candidate for lung cancer gene therapy strategies. |
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| Keywords: | pulmonary carcinoma adenovirus suicide gene gene therapy |
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