贝母类药材随机引物扩增多态性分析研究

目的探讨贝母类药材不同居群间遗传变异大小,为贝母的种质鉴定及遗传多样性分析提供依据。方法运用随机扩增多态性DNA技术,对浙江象山、浙江磐安、吉林通化、新疆伊犁四个不同居群贝母的基因组DNA进行随机扩增,利用NTsys2.10e软件计算遗传相似性,运用UPGMA法进行聚类分析并构建树状图。结果共筛选了70个随机引物,从中挑选出13条多态性强、重复性好的引物,总共检测出152个位点,多态性位点96个,多态位点比率为63.2%,UPGMA聚类可以将不同来源的贝母很好地区分开来。结论不同居群间的贝母遗传差异与地域差异有明显关系,RAPD分子标记方法可以用来鉴定不同产地的贝母。

Studies on RAPD Polymorphism of Fritillaria Herbs from Different Populations

Objective To explore genetic variation of herbs fritillaria in different populations, and to providethe basis for the identification and analysis of genetic diversity of fritillaria. Methods DNA fingerprints offritillaria in four different populations of Zhejiang xiangshan, Zhejiang panan, Jilin tonghua, Xinjiang yili wereresearched by RAPD method. Genetic similarity and dendrogram of RAPD cluster among four populations werealso analyzed by NTsys2.10e software. Results Thirteen primers were selected from total seventy primers toproduce highly reproducible RAPD bands. A total of 152 bands with 96 polymorphism bands were amplifiedfrom four populations. The ratio of genetic polymorphism was 63.2%. The DNA molecular dendrogram wasalso established by UPGMA analysis. Conclusion High genetic diversity of genetic variation occurs in differentpopulations of fritillaria. The RAPD molecular markers can be used to identify the different populations offritillaria.