非小细胞肺癌外周血CD44和CD54表达的定量研究

目的 探讨非小细胞肺癌患者外周血淋巴细胞中CD44和CD54表达与临床病理的关系。方法 应用流式细胞术对50例肺癌患者外周血淋巴细胞中CD44和CD54表达与临床病理的关系。方法 应用流式细胞术对50例肺癌患者外周血淋巴细胞中CD44和CD54表达进行荧光免疫检测，并与正常对照组（30名）及肺部良性病变组（25例）进行对比研究。结果 50例肺癌患者外周血淋巴细胞中CD44和CD54表达明显高于正常对照组及良性病变组（P＜0．01）。良性病变组和正常对照组之间CD44和CD54的表达比较，差异无显著性（P＞0．05）。肺癌伴淋巴结转移CD44和CD54高于不伴淋巴结转移者（P＜0．01）；Ⅲ期、Ⅳ期和Ⅰ期、Ⅱ期之间CD44表达比较，差异有显著性（P＜0．01）；Ⅳ期和Ⅰ期、Ⅱ期、Ⅲ期之间CD54表达比较，差异有显著性（P＜0．01）。CD44和CD54表达与肺癌组织学分级有明显相关性（P＜0．05或0．01）；与鳞癌和腺癌没有相关性。结论 应用流式细胞仪检测CD44和CD54的表达水平可作为肺癌转移和预后的指标。

Quantitative studies on expression of peripheral blood CD44 and CD54 in non-small cell lung carcinoma

OBJECTIVE: To assess the relationship between expression of peripheral blood CD44 and CD54 in lymphocyte with non-small cell lung cancer and clinicopathological features. METHODS: Peripheral blood CD44 contents in 50 patients with lung cancer were detected by flow-cytometry, 30 normal donors and 25 patients with benign lesions served as controls. RESULTS: Peripheral blood CD44 and CD54 in lymphocyte with lung cancer were significantly higher than those in benign lesions and normal controls (P < 0.01). There is no distinct difference between CD44 and CD54 in benign lesions and normal controls (P > 0.05). Lung cancer with lymph node metastasis showed higher expression of CD44 and CD54 than lung cancer without lymph node metastasis (P < 0.01). Distinctly statistic difference was showed between CD44 in stage III, IV and stage I, II (P < 0.01). CD54 content in stage IV was higher than that in stage I, II and III, respectively. Histopathological grade was significantly associated with the expression of CD44 and CD54. Peripheral blood CD44 and CD54 contents were not related with squamous cell carcinoma and adenocarcinoma. CONCLUSION: Detection of CD44 and CD54 levels by flow cytometry might be used as a marker to evaluate prognosis and metastasis in patients with non-small cell lung cancer.