Notch信号分子在人NK细胞淋巴瘤细胞中的表达及其意义

目的：探讨Notch信号分子及下游靶基因在人NK/T细胞淋巴瘤、NK细胞淋巴瘤、T细胞淋巴瘤和B细胞淋巴瘤细胞中的表达，阐明Notch信号分子在NK细胞肿瘤中的作用及意义。方法：选择NK/T细胞淋巴瘤细胞SNK-6、NK细胞淋巴瘤细胞YTS、人急性T淋巴细胞白血病细胞Jurkat和人Burkitt’s淋巴瘤细胞 Raji，通过实时荧光定量PCR技术检测上述细胞中Notch信号分子的表达水平。结果：Notch 1,3,4信号分子在YTS和SNK-6细胞中表达量均不同程度高于Raji和Jurkat淋巴瘤细胞（P<0.01），但是在SNK-6中的表达量均不同程度低于在YTS细胞中的表达（P<0.01）。Notch 2信号分子在YTS、SNK-6细胞中的表达量高于Jurkat细胞（P<0.01），但是与Raji细胞比较差异无统计学意义（P>0.05）。Notch 下游靶基因Hes1的表达量，YTS细胞高于Raji和Jurkat细胞（P<0.05,P<0.01），而SNK-6细胞则低于Raji和Jurkat细胞（P<0.01）。结论：在NK细胞肿瘤细胞中Notch 信号分子表达异常，且明显区别于B、T细胞淋巴瘤细胞。

Expression of Notch signal molecules in NK/T lymphoma cells and its significance

Abstract:Objective
To investigate the expressions of Notch signal molecules and their downstream target genes in human NK/,NK,T and B lymphoma cells and to expound the effect and significanceof Notch signal molecules in NK cell tumor.Methods The expression levels of Notch signal in YTS,SNK-6,Raji and Jurkat cells were detected by Real-time PCR.Results The expression levels of Notch 1,3,4 signal molecules in YTS and SNK-6 cells were significantly higher than those in Raji and Jurkat cells（P<0.01）,but the expression levels of Notch 1,3,4 signal molecules in SNK-6 cells were lower than that in YTS cells(P<0.01).The expression levels of Notch 2 in YTS and SNK-6 cells were significantly higher than that in Jurkat cells（P<0.01）,but there was no statistically significant difference compared with Raji cells （P>0.05）.The expression level of Hes1 in YTS cells was significantly higher than those in Raji and Jurkat cells（P<0.05 or P<0.01）,but the expression level in SNK-6 cells was lower than those in Raji and Jurkat cells（P<0.01）.Conclusion Notch signal molecules express abnormally in NK cell tumor,and they are significantly different with B and T lymphoma cells.