Comparison of sensitivity of Hep-2 cells with that of HL cells against Chlamydia pneumoniae] |
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Authors: | H Yoshizawa K Dairiki T Yamazaki |
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Affiliation: | Department of Pathobiology, School of Nursing, Chiba University. |
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Abstract: | We compared the growth of Chlamydia pneumoniae, reference strain TW183 and an isolate from a Japanese infant, AC43 in HeLa229, HL and Hep-2 cells. The mean number of inclusion-forming units was significantly higher on HL cells than HeLa229 cells, when the cells were not pretreated with DEAE-dextran. When the cells were not pretreated with DEAE-dextran, Hep-2 cells had a higher mean number of inclusion-forming units and a higher yield than other cell lines. Iscove's modified Dulbecco medium enhanced growth of strain TW183 in HeLa229 cells but had a low yield of strain AC43 in Hep-2 cells. |
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