乳腺病变组织中BcL-2,p53蛋白的表达

目的　观察 Bcl- 2 ,p5 3蛋白在浸润前系列乳腺病变(包括正常乳腺组织、导管增生、不典型导管增生、小叶增生、导管原位癌 )组织中的表达 .方法　采用免疫组化方法检测15例正常乳腺组织、18例导管增生、10例不典型导管增生(atypical ductal hyperplasia,ADH)、10例小叶增生 ,45例导管原位癌 (ductal carcinoma in situ,DCIS)的 Bcl- 2和 p5 3蛋白的表达 .结果　 Bcl- 2蛋白在正常乳腺组织、导管增生、ADH、小叶增生组织中均阳性 ,而 p5 3蛋白染色阴性 .DCIS病例中 ,Bcl- 2蛋白表达阳性 34例 (76 % ) ,而 p5 3蛋白阳性 11例(2 4% ) .Bcl- 2蛋白表达与 DCIS组织学分级密切相关 (P<0 .0 1) ,6 7% DICS病例表现为 Bcl- 2 + / p5 3-表达表型 ,还有33%不同于正常的表型 (包括所有 Bcl- 2 - / p5 3+表型 )多代表差分化病例 .结论　 Bcl- 2蛋白在正常乳腺组织、导管增生、ADH、小叶增生组织中均有表达且高于乳腺 DCIS.大部分DCIS病例表现为 Bcl- 2 + / p5 3-表型 ,与正常乳腺上皮、良性增生病变一致

Expression of Bcl-2 and p53 protein in breast lesions

AIM Expressions of Bcl 2 and p53 protein were studied in various breast lesions(including normal breast tissue,ductal hyperplasia,atypical ductal hyperplasia(ADH),lobular hyperplasia and ductal carcinoma in situ (DICS). METHODS By immunohistochemistry Bcl 2 and p53 expression were studied in cases of normal breast tissue( n =15),ductal hyperplasia(n=18),atypical ductal hyperplasia(n=10),lobular hyperplasia(n=10) and ductal carcinoma in situ (n=45). RESULTS Bcl 2 protein was expressed in the epithelial cells in all normal breast tissue,ductal hyperplasia,ADH,lobular hyperplasia,and no immunoreactivity for the p53 protein was seen in these lesions. Bcl 2 expression was present in 34(76%) of 45 DCIS cases. However,11(24%)of the DCIS cases were p 53 positive. Bcl 2 protein expression was related to the histologic grade of DCIS(P<0.01). The Bcl 2+/p53-phenotype was observed in 67% of the DCIS cases. In contrast,the remaining 33% of DCIS cases showed combinations of Bcl 2 and p53 protein expression,which differed from that of normal breast epithelium and the other kinds of lesions were studied. Most lesions with altered Bcl 2/p53+phenotypes, including all Bcl 2-/p53+ cases,represented examples of poorly differentiated DCIS. CONCLUSION The Bcl 2 protein is consistently expressed in normal breast epithelium, ductal hyperplasia,ADH, and lobular hyperplasia,and is found to be higher in these lesions than in breast DCIS. Among DCIS cases,Bcl 2 protein is more common in well differentiated and intermediately differentiated lesions. Most of DCIS lesions are Bcl 2+/p53-,similar to normal epithelium and benign proliferative lesions.