成人急性白血病患者骨髓中p16基因的表达

目的：研究成人急性白血病患者p16基因纯合缺失和甲基化变化与其表达之间的关系，探讨其成人急性白血病发生发展中的生物学意义，方法：分别用多重聚合酶联反应（PCR）技术及甲基化敏感限制性内切酶－PCR技术检测了71例成人急性白血病患者DNA中p16基因纯合缺失及甲基情况；应用原位杂交及免疫技术检测p16基因mRNA及p16基蛋白表达，结果：71例成人急性白血病患者中，2例检测出p16基因纯合缺失，在无p16基因纯合缺失的病例中，5例急性淋巴细胞白血病（5／26）和9例急性髓性白血病（9／43）患者测出p16基因甲基化，p16mRNA及p16蛋白的阳性率分别为70．4％（50／71例）、61．9％（44／71例），结论：在成人急性白血病的发生发展中，p16基因甲基化比p16基因纯合缺失更具有意义；p16基因表达异常与成人急性白血病的发生发展密切相关。

Expression of p16 gene in adult acute leukemia

Aim:To investigate the abnormality of p16 gene and its products in adult acute leukemia (AL) and to define its value. Methods:Bone marrow DNA obtained from 71 adults with AL(28 ALL and 43 AML) were studied with mul tiple comparative polymerase chain reaction and a restriction enzymes-PCR to detect the homozygous deletion and methyla-tion of p16 gene respectively. Hybridization in situ method and immunochemistry method were used to detect p16 mRNA and P16 protein respectively. Results: Homozygous loss of p16 gene was detected in 2 cases of B-ALL, and methylation was detected in 14 cases out of the other 69 cases without homozygous deletion of p16, which were 5 of 26 ALL and 9 of 43 AML. The ratio of positive expression of p16 mRNA and P16 protein were 70.4% (50/71 ) and 61. 9% (44/71 ) respectively. Conclusion:Methylation of p16 gene may play a more important role than homozygous deletion of p16 in the leukemogene-sis and progression of adult acute leukemia.