不同原因所致大鼠腹膜粘连的对比研究

目的 对比研究创伤、缺血及异物所致大鼠腹膜粘连的发生和发展特点。方法 288只大鼠 ,随机分成手术对照组、滑石粉致粘连组、创伤致粘连组、无水乙醇致粘连组、创伤 +无水乙醇致粘连组和结扎血管致粘连组 ,各组分别用相应的方法来处理大鼠蚓突盲端 ,处理后1、2、4、8周评定蚓突盲端与其周围组织的粘连程度 ,并取蚓突盲端组织行病理学检查。 结果 (1)不同时间点腹膜粘连程度比较 :创伤致粘连组和结扎血管致粘连组组内1、2周及2、4周间腹膜粘连程度差别均无显著性意义 (均P>0.05) ,而两组术后8周粘连程度明显轻于术后4周 (均P<0.05) ;滑石粉致粘连组、无水乙醇致粘连组及创伤 +无水乙醇致粘连组术后不同时间点间腹膜粘连程度差别均无显著性意义 (P>0.05)。 (2)不同原因腹膜粘连程度比较 :手术对照组的粘连程度低于其它各组 (均P<0.05) ;滑石粉致粘连组粘连程度均重于其它各组 (均P<0.05) ;创伤致粘连组的粘连程度比无水乙醇致粘连组和创伤 +无水乙醇致粘连组轻(均P<0.05) ,但后两组间差别无显著性意义 (P>0.05) ;结扎血管致粘连组的粘连程度与创伤致粘连组相仿 (P>0.05) ;(3)粘连局部病理学改变 :光镜下滑石粉致粘连组以异物肉芽肿反应及纤维组织增生为主要表现 ,而其它各组主要以非特异性炎症反应及纤维

Comparative study on peritoneal adhesions resulted from various causes in rats

Objective To comparatively study the formative and developmental characteristics of peritoneal adhesions resulted from wound, ischemia and foreign material.Methods Two hundred eighty eight Sprague-Dawley rats were randomly divided into six groups: the pseudo-operation group (control), talc-resulted adhesion group, wound-resulted adhesion group, alcohol-resulted adhesion group, wound & alcohol-resulted adhesion group and vascular ligation-resulted adhesion group. The caecums of vermiform processes were treated with corresponding methods in each group. After 1, 2, 4, 8 weeks of the treatments, the adhesions were graded blindly and the caecums of vermiform processes were resected for light microscopy examination. Results (1)comparisons within groups In the wound-resulted and vascular ligation-resulted adhesion groups, there were no significant differences in adhesive degrees between the 1st and 2nd week as well as 2nd and 4th week(P>0.05), but the adhesive grades decreased significantly at the 8th week when compared with those at the 4th week(P<0.05). There were no apparent differences in adhesive degree among the different time points in the talc-resulted, alcohol-resulted and wound & alcohol-resulted adhesion groups (P>0.05).(2)comparisons among groups At each time point after the treatments, there was significant difference in adhesive degree among the six groups(P<0.05); The adhesive degree of the control group was significantly lower than those of all experimental groups(P<0.05); The degree of talc-resulted adhesion group was the highest among the six groups. The degree of wound-resulted adhesion group was lower than those of alcohol-resulted and wound & alcohol-resulted adhesion groups (P<0.05), but there was no significant difference between the latter two groups(P>0.05).(3)changes under light microscopy The main pathological changes were foreign body granuloma reaction and fibroplasias in talc-resulted adhesion group and unspecific inflammatory reactionand fibroplasias in the other experimental groups. Conclusion The peritoneal adhesions resulted from different causes have their own characteristics in their formative and developmental courses.