nm23—H1基因在大肠癌中的表达与肝转移及预后的关系

目的：探讨ｎｍ２３－Ｈ１蛋白表达与大肠癌肝转移及预后的关系。方法：对１０１例大肠癌存档石蜡块进行重新切片，采用ｎｍ２３－Ｈ１单克隆抗体进行免疫组化染色（ＬＳＡＢ法）。结果：ｎｍ２３－Ｈ１蛋白表达与年龄、性别、肿瘤大小、部位、组织类型、浆膜侵犯无关；与Ｄｕｋｅｓ分期、淋巴结转移有关；手术时有肝转移组较无肝转移组低，手术后有肝转移复发组较无肝转移复发组低（Ｐ＜００１）。Ｃｏｘ模型分析显示ｎｍ２３－Ｈ１是大肠癌预后的一个保护性指标。结论：ｎｍ２３－Ｈ１基因对大肠癌肝转移和预后具有重要作用。ＬＳＡＢ法检测大肠癌组织中ｎｍ２３－Ｈ１蛋白表达可能是预测大肠癌肝转移及预后的生物学指标之

Expression of nm23-H1 gene associated with hepatic metastasis and prognosis in colorectal carcinoma

Objective: To evaluate the relationship of expression of nm23-H1 protein to hepatic metastasis and prognosis in colorectal carcinoma. Methods: Using labelled streptavidin-biotin (LSAB) immunohistochemical technique, we tested the expression of nm23-H1 on formalin-fixed, paraffin embedded sections of colorectal carcinomas from 101 patients. Results: There was no significant relation between the expression of nm23-H1 protein and clinicopathological findings such as sex, age, size,tumor locativn, histological type and serosal invasion. The significant relation was seen in Dukes'stage, lymph node metastasis. The expression of nm23-H1 protein in patients with hepatic metastasis, both at and after operations, were lower than those without liver metastasis (P<0.01). The Cox's regression analysis showed that nm23-H1 was a protective factor of prognosis in colorectal carcinoma. Conclusion: Immunohistochemical evaluation of nm23-H1 protein in primary tumors may be a reliable biologic marker for predicting hepatic metastasis and prognosis in colorectal carcinoma.