Molecular cloning of a beta-glucosidase-encoding gene from Sclerotinia sclerotiorum by expression in Escherichia coli

Summary Six Sclerotinia sclerotiorum genes previously described as being expressed specifically during the induction of cell-wall-degrading enzymes have been transferred to Escherichia coli. When tested for expression of beta-glucosidase activity using X-glu (5-bromo-4-chloro-3-indolyl-beta-d-glucopyranoside), one of of the the genes expressed a X-glu-degrading activity. The corresponding RNA size is reported.