Adenovirus-mediated delivery of antisense gene to urokinase-type plasminogen activator receptor suppresses glioma invasion and tumor growth. |
| |
| Authors: | P M Mohan S K Chintala S Mohanam C L Gladson E S Kim Z L Gokaslan S S Lakka J A Roth B Fang R Sawaya A P Kyritsis J S Rao |
| |
| Affiliation: | Department of Neurosurgery, The University of Texas, M.D. Anderson Cancer Center, Houston 77030, USA. |
| |
| Abstract: | The urokinase-type plasminogen activator (uPA) and uPA receptor (UPAR) play important roles in the proteolytic cascade involved in the invasiveness of gliomas and other invasive tumors. High-level expression of uPAR has been correlated with high-grade glioma cell lines and tumors We report here that down-regulating uPAR levels by antisense strategy using an adenovirus construct (Ad-uPAR) inhibited glioma invasion in Matrigel and spheroid in vitro models. sc. (U87-MG) and intracranial (SNB19) injections of Ad-uPAR-infected glioma cells did not produce tumors in nude mice. However, injection of the Ad-uPAR construct into previously established so U87-MG tumors in nude mice caused regression of those tumors. Our results support the therapeutic potential of targeting the uPA-uPAR system for the treatment of gliomas and other cancers. |
| |
| Keywords: | |
|
|
