真菌提取物JN219抗病毒谱的初步研究

目的：探讨真菌提取物JN219的体外抗病毒谱。方法：采用体外细胞病变法，研究JN219对疱疹病毒科病毒（HCMV、HSV-1、HSV-2）、腺病毒科病毒（Ad3）、副粘病毒科病毒（RSV）、呼肠病毒科病毒（RV SA11）、正粘病毒科病毒（流感甲地方株、流感乙地方株）、小RNA病毒科病毒（COX B1-6）、弹状病毒科病毒（VSV）共15株病毒的抗病毒活性。将JN219与以上病毒作用1h后接种于相应宿主细胞（同时设有病毒对照和细胞对照），当病毒对照病变达到90％以上时终止培养，宿主细胞用1％中性红染色，在450nm处读取吸光度A值，计算细胞存活率、半数中毒浓度（TD₅₀）、半数有效浓度（IC₅₀）和抑毒指数（TI）。并根据TI判断JN219的抗病毒活性，确定其抗病毒谱。结果：JN219对HCMV、RV（SA11）、VSV有抑制作用，其IC50分别为1.42、0.96和1.22μg/ml，TI分别为35.26、52.30、40.82；对HSV-1、HSV-2、RSV、流感病毒甲、流感病毒乙有部分抑制作用；对COX B1-6、腺病毒（Ad3）无作用。结论：JN219抗病毒谱比较广泛。

Antivirus spectra of JN219 extracted from certain strains of fungi

Objective： To investigate the antivirus spectra of singlet compound named JN219 extracted from certain strains of fungi. Methods： Fifteen different strains of viruses， including herpesviridae virus（HCMV, HSV-1, HSV-2）, adenoviridae virus（Ad3）, paramyxoviridae virus（RSV）, reoviridae virus(RV SA11), orthomyxoviridae（influenza A, influenza B）, picornaviridae（COX B1-6）and rhabdoviridae（VSV）were used for in-vitro antivirus experiments, and the antiviral activity and the cytotoxicity of JN219 were evaluated by the cytopathic effect(CPE) method. Infected cells were dyed with 1％ neutral red, and the decolored and the absorptance A were read by an enzyme label meter. The cell survival rate, the median toxic concentration, the median inhibiting concentrations and therapeutic indexes were calculated by the Reed-Muench method. Antivirus stability experiments of JN219 were carried out at the same time. Results： Cytopathic effects caused by HCMV, VSV and RV SA11 were significantly inhibited. The median inhibiting concentrations (IC₅₀) were 1.42, 0.96 and 1.22μg/ml, respectively, and the therapeutic indexes (TI) were 35.26, 52.30 and 40.82, respectively. HSV-1， HSV-2， RSV， influenza A and influenza B were partially inhibited, however COX B1-6 and Ad3 were not inhibited. The cytotoxicity of the samples appeared only in the first dilution and the antiviral activity was unstable. Conclusion: JN219 has a wide antivirus spectra.