| 大鼠脑缺血时紧密连接相关蛋白occludin和claudin-5表达的变化 |
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| 引用本文： | 王静娥,田国萍,周进,李丽,朱峰,徐冰,刘云会.大鼠脑缺血时紧密连接相关蛋白occludin和claudin-5表达的变化[J].解剖科学进展,2010,16(2):149-152. |
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| 作者姓名： | 王静娥 田国萍 周进 李丽 朱峰 徐冰 刘云会 |
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| 作者单位： | 1. 沈阳市第一人民医院神经内科,辽宁,沈阳,110041
2. 中国医科大学附属盛京医院神经外科,辽宁,沈阳,110004 |
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| 基金项目： | 国家自然科学基金(No.30670723,30872656,30973079);;辽宁省自然科学基金(No.20052102,20082102);;沈阳市科学技术计划基金(No.1091175-1-01,1081266-9-00,1081317-9-00) |
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| 摘 要： | 目的研究脑缺血后血脑屏障通透性和紧密连接相关蛋白occludin和claudin-5的变化。方法线栓法制备大鼠大脑中动脉缺血模型,采用伊文氏兰(EB)法检测缺血后血脑屏障通透性的变化。应用免疫组织化学的方法观察occludin和claudin-5在缺血脑组织中的分布,采用RT-PCR、Wesrern blot法检测大鼠缺血脑组织occludin和claudin-5的mRNA和蛋白的表达变化。结果脑缺血2h后血脑屏障通透性显著增加(P0.01),紧密连接相关蛋白occludin和claudin-5在脑微血管内皮细胞上呈阳性表达,occludin和claudin-5的mRNA和蛋白均比正常对照组显著降低(P0.01)。结论脑缺血时血脑屏障通透性的增加可能与紧密连接相关蛋白occludin和claudin-5的降低相关。
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| 关 键 词： | 脑缺血 血脑屏障 紧密连接 occludin claudin-5 大鼠 |
Expression changes of the tight junction-associated proteins occludin and claudin-5 in the microvessel endothelia of cerebral ischemia rat |
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| WANG Jing-e,TIAN Guo-ping,ZHOU Jin,LI Li,ZHU Feng,XU Bing,LIU Yun-hui.Expression changes of the tight junction-associated proteins occludin and claudin-5 in the microvessel endothelia of cerebral ischemia rat[J].Progress of Anatomical Sciences,2010,16(2):149-152. |
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| Authors: | WANG Jing-e TIAN Guo-ping ZHOU Jin LI Li ZHU Feng XU Bing LIU Yun-hui |
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| Institution: | 1.Department of Neurology;First People's Hospital of Shenyang City;Liaoning Shenyang 110041;2.Department of Neurosurgery;Shengjing Affliated Hospital of China Medical University;Liaoning Shenyang 110004 China |
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| Abstract: | Objective To investigate the changs of the permeability of BBB and the expression of the tight junction-associated proteins occludin and claudin-5 in the microvessel endothelia of cerebral ischemia rat.Methods Rats were scheduled to undergo middle cerebral artery ischemia by intraluminal suture.The change of the permeability of BBB was detected using evans blue(EB)dyeing.The distribution of occludin and claudin-5 was observed by the application of immunohistochemistry,and the changes of mRNA and protein exp... |
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| Keywords: | ischemia blood-brain barrier tight junction occludin claudin-5 rat |
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