Ad5F35-LMP2重组腺病毒修饰DC体外诱导特异性T细胞免疫的研究

目的 了解含有EB病毒潜伏膜蛋白2的非复制型重组腺病毒(AdSF35-LMP2)修饰的DC能否在体外诱导LMP2特异性细胞毒性T淋巴细胞免疫.方法 人外周血单个核细胞在细胞因子诱导下生成树突状细胞,Ad5F35-LMP2感染树突状细胞后激发同源的T细胞,MTT法检测其对T细胞的增殖作用,及激活的特异性CTL对表达LMP2的人CNE-2细胞的特异性杀伤效应.结果Ad5F35-LMP2能有效感染树突状细胞.其激活的特异性CTL对CNE-2细胞有特异性杀伤活性,与对照组相比差异有统计学意义(P<0.01).结论 重组腺病毒Ad5F35-LMP2感染的DC可以有效的诱导产生EBV-LMP2特异性细胞毒效应.

Dendritic cells transfected with recombinant adenovirus Ad5F35-LMP2 induces LMP2 specific immunity mediated by cytotoxic T lymphocytes in vitro

Objective To observe whether dendritic cells(DCs) transfected with recombinant adenovirus AdSF35-LMP2 induces LMP2 specific immunity mediated by cytotoxic T lymphocytes in vitro. Methods Dendritic cells have been generated in vitro, and cocultured with autologous T cell after the DCs were infected with Ad5F35-LMP2, then the proliferation of the induced T cells and their cytotoxic activity against CNE-2 tumor cells which express EBV-LMP2 protein on membrane were assessed by MTT method. Results The dendritic cells could be transfected with Ad5F35-LMP2 and the CTL activated by Ad5F35-LMP2-DC could effectively suppress the proliferation of CNE-2 cells compared with control groups. Conclusion The dendritic cells transfected with recombinant adenovirus Ad5F35-LMP2 showed cytotoxicity effect by activating T lymphocytes.