| 罗哌卡因对豚鼠心室肌细胞钠电流、钙电流和钾电流的影响 |
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| 作者姓名： | Ding HL Zeng YM Li XD Jiang WP Duan SM |
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| 作者单位： | Now in Physiological Laboratory of Hypoxia,Health Science Center,Shanghai Institutes for Biological Sciences,Chinese Academry of Sciences,Shanghai 200031,China,徐州医学院江苏省麻醉学重点实验室,苏州大学附属第一医院心内科实验室,苏州大学附属第一医院心内科实验室,徐州医学院江苏省麻醉学重点实验室 徐州 221002,苏州,中国 215006,苏州,中国 215006,徐州 221002 |
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| 摘 要： | 目的:研究罗哌卡因(Rop)对豚鼠心室肌细胞钠电流(Ⅰ_(Na))、L-型钙电流(Ⅰ_(Ca-L)、内向整流钾电流(Ⅰ_(Kl)及延迟整流钾电流(I_K)的影响.方法:全细胞膜片箝技术.结果:罗哌卡因10,50与100μmol/L使Ⅰ_(Na)的峰电流分别减小8.3％、33.3 ％和62.5％(P<0.01),使失活时间常数分别延长8.2％、24.7％和64.1％(P<0.05);罗哌卡因50与100μmol/L使Ⅰ_(Ca-L)的峰电流分别减小7.6％和22.5％(P<0.05),使慢失活时间常数分别延长15.5％和33.0％(P<0.01);罗哌卡因50与100μmol/L对Ⅰ_(Kl)和Ⅰ_K的峰电流无明显影响.结论:罗哌卡因抑制Ⅰ_(Na)和Ⅰ_(Ca-L),可能与其心脏毒性作用有关.
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| 关 键 词： | 罗哌卡因 膜片箝技术 心肌 钠通道 钙通道 钾通道 |
Effects of ropivacaine on sodium, calcium, and potassium currents in guinea pig ventricular myocytes |
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| Ding HL,Zeng YM,Li XD,Jiang WP,Duan SM.Effects of ropivacaine on sodium, calcium, and potassium currents in guinea pig ventricular myocytes[J].Acta Pharmacologica Sinica,2002,23(1):50-54. |
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| Authors: | Ding Hai-Lei Zeng Yin-Ming Li Xiao-Dong Jiang Wen-Ping Duan Shi-Ming |
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| Institution: | Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221002, China. hailei0314@263.net |
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| Abstract: | AIM: To study the effects of ropivacaine (Rop) on sodium current (INa), L-type calcium current (ICa-L), inward rectifier potassium current (IK1), and delayed rectifier potassium current (IK) in isolated guinea pig ventricular myocytes. METHODS: Whole cell patch-clamp techniques were used in our experiment. RESULTS: At potential of -40 mV, Rop 10, 50, and 100 micromol/L decreased sodium current by 8.3 %, 33.3 %, and 62.5 %, respectively and prolonged the time constant of INa inactivation by 8.2 %, 24.7 %, and 64.4 %, respectively (n = 5 cells from 3 animals, P < 0.05). At potential of +10 mV, Rop 50 and 100 micromol/L decreased L-type calcium current by 7.6 % and 22.5 %, and prolonged the slow time constant of ICa-L inactivation by 15.5 % and 33.0 %, respectively (n = 5 cells from 4 animals, P < 0.05). Rop 50 and 100 micromol/L did not markedly change the peak current of delayed rectifier potassium current and inward rectifier potassium current (n = 5 cells from 3 animals, P > 0.05), respectively. CONCLUSION: Rop depressed INa and ICa-L, which may be related to its cardiotoxic effect |
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| Keywords: | ropivacaine patch-clamp techniques myocardium sodium channels calcium channels potassium channels |
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