CREPT对胶质瘤U251细胞增殖、侵袭和迁移的影响及机制

目的 探讨肿瘤高表达细胞周期相关蛋白（CREPT）对胶质瘤U251细胞增殖、侵袭和迁移的影响及可能机制。方法 体外培养人胶质瘤细胞株U373、U251、A172、U87-MG、SHG44，并构建过表达或沉默CREPT的U251细胞；免疫印迹法检测蛋白表达水平；CCK-8法检测细胞增殖能力；Transwell实验检测细胞侵袭能力；细胞划痕实验检测细胞迁移能力。结果 U251细胞CREPT蛋白表达水平最高，SHG44细胞最低。沉默CREPT后，U251细胞增殖、侵袭和迁移能力明显下降（P<0.05），p-Wnt和p-β-catenin蛋白表达水平下降（P<0.05）。过表达CREPT后，U251细胞增殖、侵袭和迁移能力明显增强（P<0.05），p-Wnt和p-β-catenin蛋白水平明显升高（P<0.05）。Wnt/β-catenin通路抑制剂KYA1797K可逆转过表达CREPT对细胞增殖、侵袭和迁移的影响（P<0.05）。结论 CREPT可能通过激活Wnt/β-catenin通路促进胶质瘤U251细胞的增殖、侵袭和迁移。

Effect of CREPT on proliferation,invasion and migration of glioma U251 cells

Objective To investigate the effect of tumor overexpression of cell cycle associated protein (CREPT) on the proliferation, invasion and migration of glioma U25 cells and its mechanism. Methods Human glioma cell lines U373, U251, A172, U87-MG, and SHG44 were cultured in vitro. U251 cells with overexpressing or silencing CREPT were constructed. Western blotting was used to detect the protein expression levels of CREPT, p-Wnt and p-β-catenin. CCK-8 method was used to detect the cell proliferation. Transwell cell migration assay was used to detect the cell invasion ability. Cell scratch test was used to detect the cell migration ability. Results The CREPT protein expression level was highest in U251 cells, and lowest in SHG44 cells. After silencing CREPT, the proliferation, invasion and migration abilities of U251 cells decreased significantly (P<0.05), and the expression levels of p-Wnt and p-β-catenin protein decreased significantly (P<0.05). After overexpression of CREPT, the proliferation, invasion and migration capabilities of U251 cells were enhanced significantly (P<0.05), and the protein levels of p-Wnt and p-β-catenin increased significantly (P<0.05). Wnt/β-catenin pathway inhibitor KYA1797K could reverse the effect of overexpression of CREPT on the proliferation, invasion and migration of U251 cells (P<0.05). Conclusion CREPT may promote the proliferation, invasion and migration of glioma U251 cells by activating the Wnt/β-catenin pathway.