食源性原花青素对SH-SY5Y细胞生长的抑制作用及其机制

目的：探讨食源性原花青素对人神经母细胞瘤SH-SY5Y细胞生长的抑制作用，并阐明其作用机制。方法：培养SH-SY5Y细胞，将细胞分为对照组及10、20和40mg·L^-1食源性原花青素组，各组细胞中加入含不同浓度（0、10、20和40mg·L^-1）食源性原花青素的培养基，采用噻唑蓝（MTT）法检测药物作用24、48和72h时SH-SY5Y细胞增殖率，流式细胞术检测药物作用72 h时不同细胞周期SH-SY5Y细胞百分率，Annexin Ⅴ凋亡试剂盒检测药物作用72 h时SH-SY5Y细胞的凋亡率。结果：与对照组比较，各时间点10、20和40mg·L^-1食源性原花青素组SH-SY5Y细胞增殖率均降低，作用48和72h时20mg·L^-1食源性原花青素组细胞增殖率差异有统计学意义（P<0.05或P<0.01），作用24、48和72h时40mg·L^-1食源性原花青素组细胞增殖率差异有统计学意义（P<0.01）。与对照组比较，40mg·L^-1组食源性原花青素组细胞中G₀/G₁期细胞百分率升高（P<0.01），G₂/M期细胞百分率降低（P<0.01）。与对照组比较，10、20和40mg·L^-1食源性原花青素组细胞凋亡率均明显升高（P<0.01）。结论：食源性原花青素可抑制人神经母细胞瘤SH-SY5Y细胞的生长，其机制主要是阻滞细胞周期和诱导细胞凋亡。

Inhibitory effect of foodborne procyanidins on growth of SH-SY5Y cells and its mechanism

Objective: To investigate the inhibitory effect of foodborne procyanidins on the growth of human neuroblastoma SH-SY5Y cells, and to elucidate its mechanism. Methods: The SH-SY5Y cells were cultured and divided into control group, 10 mg·L^-1 foodborne procyanidins group, 20 mg·L^-1 foodborne procyanidins group and 40 mg·L^-1 foodborne procyanidins group;the medium containing different concentrations (0, 10, 20 and 40 mg·L^-1) of foodborne procyanidins was added into each group. The proliferation rates of SH-SY5Y cells were measured by MTT method at 24, 48 and 72 h after the drug treatment. Flow cytometry was used to detect the cell cycle of SH-SY5Y cells at 72 h after the drug treatment, and the apoptotic rate of SH-SY5Y cells was detected by Annexin Ⅴ apoptotic assay kit at 72 h after the drug treatment. Results: Compared with control group, the proliferation rates of SH-SY5Y cells at 24, 48 and 72 h in 10, 20 and 40 mg·L^-1 foodborne procyanidins groups were decreased; there were significant differences at 48 and 72 hours in 20 mg·L^-1 foodborne procyanidins group (P<0.05 or P<0.01);there were also significant differences at 24, 48 and 72 h in 40 mg·L^-1 foodborne procyanidins group (P<0.01). Compared with control group, the percentage of cells in G₀/G₁ phase in 40 mg·L^-1 foodborne procyanidins group were increased(P<0.01) and the percentage of cells in G₂/M phase were decreased (P<0.01). Compared with control group, the apoptotic rates of cells in 10, 20 and 40 mg·L^-1 foodborne procyanidins groups were increased significantly (P<0.01). Conclusion: Foodborne procyanidins can inhibit the growth of human neuroblastoma SH-SY5Y cells, and its mechanism is mainly to block the cell cycle and induce the apoptosis.