孤独症谱系障碍儿童肠道菌群多样性与Treg-Th17的相关性研究

目的 调查分析孤独症谱系障碍(atism spectrum disorder,ASD)儿童肠道菌群多样性情况,探究其与Treg-Th17的相关性。 方法 选择2016年10月—2019年4月金华市中心医院49例孤独症谱系障碍患儿纳入研究(ASD组),另选50例健康体检儿童为健康对照组。测定外周静脉血单个核细胞(PBMC)内Treg、Th17含量及Treg/Th17比值,经ELISA检测血清TGF-β1、IL-17指标,观察肠道菌群多样性与Treg-Th17相关性。 结果 ASD组肠道内双歧杆菌、大肠埃希菌基因拷贝数及B/E值低于健康对照组(均P<0.05);ASD组PBMC内Treg细胞数量、Treg/Th17值低于健康对照组,但PBMC内Th17细胞数量高于健康对照组(均P<0.05);ASD组血浆IL-17[(311.28±29.83)ng/L]高于健康对照组[(104.96±17.51)ng/L],血浆TGF-β1水平[(28.33±8.14)ng/L]低于健康对照组[(101.84±12.76)ng/L,t=42.070、34.095,均P<0.05]。经Pearson相关性分析发现,肠道B/E值和Treg、Th17及Treg/Th17值呈正相关(r=0.522、0.416、0.323,均P<0.05)。 结论 孤独症谱系障碍患儿的肠黏膜屏障功能紊乱,主要表现为免疫屏障作用减弱、肠道微生态失衡,且肠道菌群介导性耐受异常,贯穿于孤独症谱系障碍病理过程。 

Correlation between gut microbiota diversity and Treg-Th17 in children with autism spectrum disorder

Objective To investigate and analyze the diversity of intestinal flora in children with autism spectrum disorder(ASD), to explore its correlation with Treg-Th17. Methods Forty-nine cases of children with autism spectrum disorder admitted to Jinhua Central Hospital from October 2016 to April 2019 were included in the study.Another 50 normal children who underwent physical examination in our hospital were selected as healthy controls. The content of Treg, Th17 cells and Treg/Th17 in peripheral venous blood PBMC(mononuclear cells) were measured by flow cytometry and detected by ELISA method. The serum levels of TGF-β1 and IL-17 in children were observed to correlate the diversity of intestinal flora in ASD with Treg-Th17. Results The number of Bifidobacterium and Escherichia coli gene copies and B/E values in the intestinal tract of the ASD group were lower than those of the healthy control group(all P<0.05). The number of Treg cells and Treg/Th17 value in PBMC of ASD group were lower than those of healthy control group, but the number of Th17 cells in PBMC was higher than that of healthy control group(all P<0.05). The plasma IL-17[(311.28±29.83) ng/L] in the ASD group was higher than that in the healthy control group[(104.96±17.51) ng/L], and the plasma TGF-β1 level[(28.33±8.14) ng/L] was lower than that in the healthy control group[(101.84±12.76) ng/L, t=42.070, 34.095, all P<0.05]. Pearson correlation analysis found that intestinal B/E value and Treg, Th17 and Treg/Th17 values showed a positive correlation(r=0.522,0.416,0.323, all P<0.05). Conclusion The intestinal mucosal barrier dysfunction in children with acute autism spectrum disorder is mainly due to weakened immune barrier, intestinal micro-ecological imbalance, and intestinal flora-mediated tolerance, which runs through the pathological process of autism spectrum disorder.