黑果腺肋花楸提取物的急性毒性和致突变性研究

目的:检测黑果腺肋花楸提取物的急性毒性和潜在的致突变性。方法:小鼠按20 g/kg灌胃给予黑果腺肋花楸提取物溶液,检测其急性经口毒性;采用细菌回复突变试验、小鼠骨髓细胞微核试验和精子畸形试验检测其致突变性。结果:在给予20 g/kg的黑果腺肋花楸提取物(原花青素的浓度为558 mg/kg)后,小鼠未出现中毒体征和死亡;在加与不加S9混合液的条件下,8、40、200、1000、5000μg/皿5个浓度组的4种菌株的回复突变菌落数与自发回复突变菌落数相近,MR值均小于2;2.5、5、10 g/kg 3个浓度组的微核率和精子畸形率与阴性对照组的差异均无统计学意义(P>0.05)。结论:在本实验条件下,未发现黑果腺肋花楸提取物对实验动物有急性经口毒性和致突变作用。

Study on acute toxicity and mutagenicity of Aronia melanocarpa extract in mice

OBJECTIVE: To determine acute toxicity and mutagenicity of Aronia melanocarpa extract. METHODS: The Aronia melanocarpa extract solution was given orally to mice with a dose of 20 g/kg to determine its acute oral toxicity. In addition,mutagenicity was assessed by a bacterial reverse mutation assay,micronucleus test and sperm shape abnormality test. RESULTS: The oral maximum tolerated dose (MTD) of Aronia melanocarpa extract in mice was more than 20 g/kg. No increase in the number of revertant colonies was found in the bacterial reverse mutation assay with or without S9 mixture in 8,40,200,1 000,5 000 μg/plate of Aronia melanocarpa extract. The micronucleus rates and sperm abnormality rates in 2.5,5,10 g/kg dose were not significantly different from the negative control. CONCLUSION: This Aronia melanocarpa extract showed no acute oral toxicity and nor mutagenicity in mice.