NFKBIA基因多态性、饮食习惯及其交互作用与胃癌易感性的关系

目的:分析NFKBIA基因多态性、饮食习惯及其交互作用与胃癌易感性的关系。方法:采用1∶1配对病例对照研究设计,收集2013年4月-2017年6月福建仙游县医院587例胃癌新发病例和按性别、年龄匹配的587例健康人群为研究对象,采用自制调查表收集一般情况、饮食习惯等资料,利用Sequenom Mass ARRAY SNP方法检测外周血NFKBIA基因rs696位点的基因型。应用条件logistic回归模型分析饮食习惯、基因型对胃癌发病风险的影响,利用叉生分析结合logistic回归模型和Andersson等编制的Excel表进行基因与环境的交互作用分析。结果:快速饮食、不规律进餐、高盐饮食和经常摄入腌菜是胃癌发生的危险因素,OR(95%CI)分别为1.53(1.20,1.95)、1.55(1.16,2.08)、1.51(1.17,1.94)、2.78(2.01,3.85);NFKBIA基因rs696位点携带AG基因型、AA基因型、显性模型(AG+AA)增加胃贲门癌发病风险。此外,rs696位点AA基因型与高盐饮食、经常摄入腌菜存在联合作用,rs696位点显性模型(AG+AA)与快速饮食、高盐饮食、经常摄入腌菜存在联合作用,均增加胃贲门癌发病风险。结论:不良饮食习惯是胃癌发生的危险因素,NFKBIA基因rs696位点AA基因型、显性模型(AG+AA)增加胃贲门癌发病风险,且与饮食习惯存在联合作用。

NFKBIA polymorphism,eating habits and susceptibility to gastric cancer: a case-control study

OBJECTIVE: To analyze relationships between NFKBIA gene polymorphism,eating habits and susceptibility to gastric cancer. METHODS: 1:1 matched case-control study was carried out with 587 newly-diagnosed primary gastric patients and 587 controls matched by sex and age in the Fujian Xianyou hospital from April 2013 to June 2017. The data of general information and eating habits were collected by a self-made questionnaire. The Sequenom MassARRAY SNP detection method was used to detect the genotype of NFKBIA gene rs696 locus. Condition logistic regression was used to analyze the effects of eating habits and genotypes on risk of gastric cancer. Forked analysis,logistic regression model and Excel table compiled by Andersson were used to evaluate the interactive effects of NFKBIA polymorphism and eating habits. RESULTS: Fast eating,irregular meals,high salt diet and often pickle intakes were the risk factors of gastric cancer. OR(95%CI) were 1.53(1.20,1.95),1.55(1.16,2.08),1.51(1.17,1.94),2.78(2.01,3.85) respectively. The NFKBIA rs696 locus AG genotype,AA genotype and dominant model (AG+AA) increased the risk of gastric cardia cancer. In addition,the interaction effects were found on rs696 AA genotype with high salt diet and pickle intakes. The interactive effects were also found on the rs696 dominant model (AG+AA) with fast eating,high salt diet and often intake pickles. CONSLUSION:Bad eating habit was a risk factor of gastric cancer. The NFKBIA rs696 AA genotype,the dominant model (AG+AA) increased the risk of gastric-cardia cancer and had an interaction effect with eating habits.