Capto Core 400和Sepharose 6FF在Sabin株脊髓灰质炎病毒纯化工艺的应用与比较

目的 分别用新型复合型层析介质Capto Core 400和传统介质Sepharose 6FF纯化Sabin株脊髓灰质炎病毒（Sabin strain poliovirus，sPV）去除宿主细胞蛋白（host cell protein，HCP）和DNA，并且比较纯化结果及工艺参数。方法  用Capto Core 400与Sepharose 6FF纯化Vero细胞培养的Ⅰ、Ⅱ、Ⅲ型sPV浓缩液。分别检测纯化后sPV的D抗原含量、HCP残留量和DNA残留量，计算D抗原回收率、HCP和DNA去除率。结果 Capto Core 400与Sepharose 6FF纯化Ⅰ、Ⅱ、Ⅲ型sPV的D抗原回收率差异均无统计学意义(t值分别为1.09、1.08、1.02,P值均>0.05)，但前者的Vero细胞HCP(t值分别为3.15、3.23、3.54)和DNA去除率均高于后者(t值分别为3.41、3.25、3.62)，且差异有统计学意义（P值均小于0.05）Capto Core 400与Sepharose 6FF的介质使用体积比为1/20，上样后纯化时间比为1/25，工作缓冲液使用量比为1/4，工艺线性流速比为10，每升介质上样量比为20，最大耐压力比为2。结论  对比Sepharose 6FF，Capto Core 400可以更有效地去除Vero细胞HCP与DNA，各个工艺参数得到了优化，提高了sPV层析纯化的工作效率，并节约时间和成本。

Application and comparison of Capto Core 400 and Sepharose 6FF purification process in Sabin strain poliovirus#br#

Objective  To purify Sabin strain poliovirus (sPV) using new multimodal chromatography medium Capto Core 400 and traditional medium Sepharose 6FF to remove residual host cell protein (HCP) and DNA, comparing purification results and process parameters. Methods  Types Ⅰ, Ⅱ, Ⅲ sPV concentrates were purified using Capto Core 400 and Sepharose 6FF media, respectively. D antigen contents, HCP and DNA residues in purified sPV liquids were detected. D antigen recovery rates, as well as HCP and DNA removal rates were caculated. Results  D antigen recovery rates of types Ⅰ, Ⅱ, Ⅲ sPV purified by Capto Core 400 and Sepharose 6FF had no statistically significant difference (t values were 1.09, 1.08, and 1.02, respectively, all P values>0.05). But HCP residue (t values were 3.15, 3.23 and 3.54, respectively) and cell DNA residue removal rates (t values were 3.41, 3.25 and 3.62, respectively) of the former were all higher than the latter, all with statistically significant differences (all P values<0.05). The specific values for Capto Core 400 and Sepharose 6FF process parameter ratios were: medium volume 1/20, purification time 1/25, application volume of buffer 1/12, process linear velocity 10, loading quantity of sample per litre medium 20, maximum withsatand pressure 2. Conclusion  Comparing with Sepharose 6FF, Capto Core 400 removes residual Vero cell HCP and DNA more effectively, with more highly optimized process parameters, resulting in increased sPV purification process efficiency and lowered time and cost.