CD10蛋白在结直肠癌组织中的表达及其临床意义

目的：研究CD10蛋白在结直肠癌组织中的表达及其与结直肠癌的各临床病理指标之间的关系，探讨其在结直肠癌发生、发展中的作用。方法：收集78例结直肠癌组织标本及22例癌旁正常组织样本，运用免疫组化EliVision法检测CD10蛋白的表达，并采用卡方检验分析其表达强度及分布比例与结直肠癌临床病理指标的关系。结果：CD10蛋白在结直肠癌组织和正常结肠黏膜组织中的阳性表达率分别为46.2%（36/78）和0，差异有统计学意义（P < 0.01）。结直肠癌组织中CD10蛋白表达比例与各临床病理指标均无明显相关（P > 0.05）。结直肠癌组织中CD10蛋白表达强度与患者性别、年龄、肿瘤部位、分化程度、浸润深度无明显相关（P > 0.05），而与肿瘤的临床分期、有无脉管侵犯及淋巴结转移有关（P < 0.05），中晚期结直肠癌CD10蛋白表达强度高于早期肿瘤（P=0.033），周围淋巴结转移阳性的结直肠癌CD10蛋白表达强度高于淋巴结阴性病例（P=0.023），存在脉管受侵犯的结直肠癌CD10蛋白表达强度高于无脉管受侵病例（P=0.004）。结论：CD10蛋白在结直肠癌组织中呈高表达，且表达强度与肿瘤的临床分期、有无脉管侵犯及淋巴结转移有关，可能有促进结直肠癌发展和浸润转移的作用。

Expression of CD10 protein in colorectal carcinomas and its clinical significance

OBJECTIVE: The aim of this study was to investigate associations between expression of CD10 protein and clinicopathological characteristics in colorectal cancer patients. METHODS: 78 cases of colorectal cancer tissue samples and 22 cases of neighboring noncancer colorectal tissue samples were collected from colorectal cancer patients. In addition,expression of CD10 protein in tissue samples was determined using rapid immunohistochemical EliVision method. Relationships between the expression intensity and clinicopathological characteristics of colorectal cancer were analyzed by the Chi Square test. RESULTS: There was no expression of CD10 protein in 22 normal colorectal tissue samples. The positive rate of CD10 expression was significantly different in colorectal cancer tissue samples and in neighboring noncancer colorectal tissue samples:46.2%(36/78) and 0,(P < 0.01). The expression intensity of CD10 protein in cancer tissues was not clearly related to gender,age,tumor location,differentiation degree and depth of invasion (P > 0.05) but was higher in the more advanced stages than in the early stages (P=0.033),and was also associated with lymph node metastasis (P=0.023) and vessel invasion (P=0.004). On the other hand,the data show no significant correlation between the expression ratio of CD10 protein and clinicopathological characteristics of colorectal cancer (P > 0.05). CONCLUSION: CD10 protein was highly expressed in colorectal cancer tissues and the expression intensity was associated with clinical stage,lymph node metastasis and vessel invasion,which suggests that CD10 protein may play roles in promoting the development,invasion and metastasis of colorectal cancer.