氮掺杂碳纳米酶联合近红外光抗真菌体外试验研究

目的:评价氮掺杂碳纳米酶(N-Carbon nanozyme)联合近红外光对白念珠菌和红色毛癣菌体外抗菌的效果。方法:将白念珠菌和红色毛癣菌标准菌株常规接种培养,分别分为四组:(1)空白对照组:真菌不作任何处理;(2)光照组:真菌予以2.5 W/cm^2近红外光照射8 min;(3)材料组:真菌予以250μg/mL的N-Carbon nanozyme处理30 min;(4)实验组:真菌予以250μg/mL的N-Carbon nanozyme及2.5 W/cm^2近红外光照射8 min。SYTO 9/PI染色判断白念珠菌和红色毛癣菌的菌体活性;扫描电镜观察和平板计数法判定白念珠菌抗菌形态和菌落形成单位(CFU)的数量。结果:实验组中SYTO9/PI染色示红色荧光染色信号显著增加;与空白对照组比较,光照组、材料组间菌落数均未见明显差异,而实验组可见菌落明显减少。生物扫描电镜显示白念珠菌的细胞形态发生明显的变化。结论:氮掺杂碳纳米酶联合近红外光对白念珠菌及红色毛癣菌均有明显的抑制作用。

Antifungal effect of nitrogen-doped carbon nanozyme combined with near infrared in vitro

Objective: To evaluate the antifungal effect of nitrogen-doped carbon nanozymes (N-carbon nanozyme) combined with near infrared on Candida albicans (C. albicans) and Trichophyton rubrum (T. rubrum). Methods: The standard strains of C. albicans and T. rubrum were routinely inoculated and cultured, and then, divided into four groups: (1) blank control group: the fungal group without treatment; (2) light group: the fungus was exposed to 2.5 W/cm2 near-infrared light for 8 min; (3) material group: the fungus was treated with 250 μg/mL N-carbon nanozyme for 30 min; (4) the experimental group: the fungus was treated with 250 μg/mL N-carbon nanozyme and 2.5 W/cm2 near infrared light for 8 min. The antifungal activity was observed by confocal fluorescence microscope after SYTO 9/PI staining. The morphological structure and the colony number of C.  albicans were detected by scanning electron microscopy and plate counting method. Results: The SYTO9/PI staining showed that the red fluorescence was strongest in the C. albicans and T. rubrum experimental groups. Plate counting method showed the CFU count in the C. albicans experimental group was significantly less than those in other groups. The biological scanning electron microscope revealed that the cell morphology was distorted significantly. Conclusion: N-carbon nanozyme combined with NIR irradiation has significant antifungal effects on C. albicans and T. rubrum in vitro.