产利普斯他汀菌株Streptomyces toxytricini FIM-17-16发酵条件的优化

目的 Streptomyces toxytricini FIM-17-16菌株生物合成利普斯他汀发酵条件的优化。方法采用单因素试验和正交设计实验相结合的方法,优化Streptomyces toxytricini FIM-17-16发酵培养基和发酵参数。结果确定了适宜发酵培养基配方和发酵参数:葵花籽油7%,甘油2%,黄豆粉3.25%,麸皮1%,卵磷脂1%,硫酸锌0.01%,碳酸钙0.08%,初始pH自然,装料系数80mL/500mL,种龄20h,接种量15%,摇床转速230r/min,发酵温度28℃,发酵时间168h,产利普斯他汀的水平达到了9667μg/mL,提高了20.6%,发酵周期缩短24h。结论经过优化发酵条件,S. toxytricini FIM-17-16生物合成利普斯他汀能力大幅提高,为该菌株的工业化生产奠定了基础。

Optimization of fermentation conditions for Streptomyces toxytricini FIM-17-16

Objective To study the optimal fermentation conditions for lipstatin production by S. toxytricini FIM-17-16. Methods The single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium and parameters of S. toxytricini FIM-17-16. Results The optimum fermentation medium and parameters were sunflower seed oil 7%, glycerine 2%, soybean flour 3.25%, bran 1%, ZnSO4 0.01%, lecithin 1.00% and CaCO3 0.08%, initial pH not adjusted, filling volume 80mL for each 500mL shake flask, cultivation time of seed 20h, inoculum size 15%, shaker’s rotating speed 230r/min, fermentation temperature 28℃, and fermentation cycle 168h. Compared with that using the original fermentation conditions, the production of lipstatin reached 9,667μg/mL under the optimization of fermentation conditions, which increased 23%, and the fermentation cycle shortened by 24h. Conclusion After optimizing the fermentation conditions, the ability of S. toxytricini FIM-17-16 to biosynthesize lipstatin has been greatly improved, laid the foundation for further industrial fermentation.