miRNA-101 Targets TGF-bR1 to Retard the Progression of Oral Squamous Cell Carcinoma |
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Authors: | Yong Wang Rui-Zhi Jia Shu Diao Jun He Li Jia |
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Institution: | * Department of Pediatric Dentistry, Beijing Stomatological Hospital & School of Stomatology,
Capital Medical University, Beijing, China† Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention of PLA, Beijing, China |
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Abstract: | Despite the considerable knowledge on the involvement of microRNA-101 (miR-101) in the evolution of oral
squamous cell carcinoma (OSCC), the underlying mechanisms remain obscure. In this study, miR-101 expression
was markedly downregulated in the OSCC cell lines and tissues. Cell counting kit-8 (CCK-8), ethynyl deoxyuridine (EdU), and colony formation assays showed that miR-101 inhibited the proliferation of OSCC cells. Flow
cytometry and caspase 3 activity assays indicated that miR-101 induced OSCC cell apoptosis. Transwell assays
demonstrated that this miRNA also repressed OSCC cell migration and invasion. Moreover, tube formation
assay showed that miR-101 abated the proangiogenesis of OSCC cells. Dual-luciferase reporter assay confirmed
that miR-101 directly targeted transforming growth factor- receptor 1 (TGF- R1) in OSCC. Ectopic expression
of TGF- R1 counteracted the effects of miR-101 on the OSCC cell characteristics. Thus, miR-101 significantly
abolished the proliferation, motility, and proangiogenesis of OSCC cells and induced their apoptosis by targeting
TGF- R1. These results imply the potential application of miR-101 in OSCC treatment. |
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Keywords: | miR-101 Transforming growth factor-b receptor 1 (TGF-bR1) Oral squamous cell carcinoma (OSCC) Growth Metastasis |
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