长链非编码RNA LINC01106通过STAT3通路调控结直肠癌细胞增殖及凋亡

目的 探讨LINC01106在结直肠癌中的表达及其对结直肠癌细胞增殖及凋亡能力的影响。方法 分析公共数据库结直肠癌患者的肿瘤组织及正常组织LINC01106表达水平及其对患者预后的影响；构建LINC01106敲减及过表达结直肠癌细胞株；采用CCK-8实验检测LINC01106敲减及过表达对结直肠癌细胞增殖能力的影响；流式细胞术检测LINC01106敲减对结直肠癌细胞凋亡水平的影响；免疫印迹检测LINC01106敲减对结直肠癌细胞p-STAT3、STAT3、Bcl-2蛋白表达的影响；建立裸鼠移植瘤模型观察LINC01106表达水平对结直肠肿瘤体内生长的影响，随机分为2组，9只/组。对照组：接种SW480细胞，敲减组：接种LINC01106敲减的SW480细胞。结果 TCGA数据库分析结果显示结直肠癌肿瘤组织LINC01106的表达水平明显高于正常组织，且LINC01106的表达水平与结直肠癌患者预后相关，差异具有统计学意义（P<0.05）；敲减LINC01106可以明显抑制SW480结直肠癌细胞增殖，促进凋亡（P<0.05），过表达LINC01106可以明显促进SW480结直肠癌细胞增殖；敲减LINC01106可以抑制p-STAT3和Bcl-2蛋白表达水平；下调LINC01106可以抑制裸鼠移植瘤体内生长。结论 LINC01106在结直肠癌患者肿瘤组织中表达显著上调，且与患者预后相关。INC01106可以通过STAT3/Bcl-2信号调控SW480结直肠癌细胞增殖及凋亡；LINC01106有望成为结直肠癌诊断及预后评估的潜在标志物。

Long non-coding RNA LINC01106 regulates colorectal cancer cell proliferation andapoptosis through the STAT3 pathway

Objective To investigate the expression of LINC01106 in colorectal cancer and its role in regulating the proliferationand apoptosis of colorectal cancer cells. Methods We analyzed the data of LINC01106 expression levels in tumor tissues andnormal tissues of patients with colorectal cancer in TCGA database and explored the association of LINC01106 expression levelwith the prognosis of the patients. Colorectal cancer SW480 cell lines with LINC01106 knockdown or overexpression wereestablished, and their proliferation and apoptosis relative to the parental cells were evaluated using CCK-8 assay and flowcytometry, respectively. The expressions of p-STAT3, STAT3, and Bcl-2 in the cells were detected by immunoblotting. Nudemouse models bearing xenografts of SW480 cells with LINC01106 knockdown or na?ve SW480 cells were established toobserve the effect of LINC01106 knockdown on the growth of SW480 cells in vivo. Results Analysis of the data from TCGAdatabase showed that the expression level of LINC01106 was significantly higher in colorectal cancer tissues than in normaltissues, and LINC01106 expression level was significantly related to the prognosis of the patients (P<0.05). Knockdown ofLINC01106 significantly inhibited the proliferation and promoted apoptosis of SW480 cells (P<0.05), while LINC01106overexpression significantly promoted proliferation of the cells. LINC01106 knockdown in SW-480 cells obviously lowered theexpressions of p- STAT3 and Bcl-2 and suppressed the growth of the xenograft in nude mice. Conclusion LINC01106 issignificantly up-regulated in colorectal cancer tissue and is related to the prognosis of the patients. LINC01106 can regulate theproliferation and apoptosis of SW480 cells through STAT3/Bcl-2 signaling and may serve as a potential marker for thediagnosis and prognostic evaluation of colorectal cancer.