HMGB1 在食管鳞癌中的表达及其意义*

目的：初步探讨食管鳞癌中高迁移率蛋白B1（HMGB 1）的表达及其与淋巴结转移和患者预后的关系。方法：收集72例有完整临床及随访资料的胸段食管鳞癌石蜡标本，采用免疫组织化学染色和统计学的方法，观察HMGB 1 和VEGF-C、VEGF-D 、LYVE-1 在食管鳞癌组织中的表达，并分析HMGB 1 与VEGF-C、VEGF-D 、微淋巴管密度（MLD）、淋巴结转移及各种临床病理参数和患者预后之间的关系。结果：HMGB 1 在食管鳞癌组织中呈高表达，阳性率达69.44% ，而在正常组织中很少表达，并且有淋巴结转移的表达率为81.82% ，无淋巴结转移的表达率为58.97% ，有显著性差异（P<0.05）。 另外，HMGB 1 的表达与食管鳞癌的分期、VEGF-C、VEGF-D 的表达、MLD及患者的预后密切相关（P<0.05或P<0.01）；同时，VEGF-C、VEGF-D 的表达也与食管鳞癌的淋巴结转移、MLD密切相关（P<0.05或P<0.01）。 结论：在食管鳞癌中，HMGB 1 的大量表达可能是通过调控VEGF-C、VEGF-D 的表达来促进新生淋巴管生成，从而促进其淋巴结转移并影响患者的预后。因此，HMGB 1 有可能作为反映食管癌预后的重要生物学指标及抗食管癌淋巴管生成的重要靶点。

Expression and Significance of HMGB1 in Esophageal Squamous Cell Carcinoma

Objective: To conduct a preliminary investigation on the expression of the high-mobility group box-1 (HMGB1) protein, and its relationship to metastasis of the lymph nodes and prognosis of patients with esophageal squa -mous cell carcinoma (ESCC).Methods:Specimens from72cases with complete clinical and follow-up data of thoracic ES -CC were collected. Immunohistochemical and statistical methods were used to observe the expressions of HMGB1 and VEGF-C, VEGF-D, LYVE-1 in ESCC. The relationship among the expression of HMGB1, VEGF-C and VEGF-D, micro-lym -phatic vessel density (MLD), lymphatic metastasis, as well as between the pathological parameters and prognosis of the ESCC patients were analyzed. Results: There was a high expression of HMGB 1 protein in ESCC, and the positive rate of the protein reached 69.44%. However, there was less expression in the normal tissue. The positive rate of HMGB1 expres-sion in the group with lymph node metastasis was significantly higher than in the group without ( 81.82% vs58.97%), with a significant difference between the two (P<0.05). Moreover, the HMGB 1 expression was closely correlated to ESCC staging, expression of VEGF-C and VEGF-D, MLD, as well as the prognosis of the patients ( P<0.05or P<0.01). At the same time, the VEGF-C and VEGF-D expression were also in close relation with the lymphatic metastasis of ESCC and MLD ( P<0.05,P<0.01) Conclusion:In ESCC, the considerable expression of HMGB 1 may promote the lymph- angiogenesis by regulating the expression of VEGF-C and VEGF-D, thus facilitating the lymphatic metastasis and affecting the prognosis. Therefore, HMGB1 may possibly become a major biological marker reflecting the prognosis of ESCC and an important target of an-ti-lymphangiogenesis in treatment.